Effect of low-power laser irradiation on cell growth and procollagen synthesis of cultured fibroblasts.

Abstract

BACKGROUND AND OBJECTIVES:

In dentistry, low-power lasers have been used in the treatment of dentin hypersensitivity, gingivitis, periodontitis, and different forms of oral ulcers. This in vitro study focuses on the biostimulation of NIH-3T3 fibroblasts by a low-power Ga-As-pulsed laser.

STUDY DESIGN/MATERIALS AND METHODS:

We have studied cell growth and procollagen synthesis of cultured fibroblasts submitted to low-power laser irradiation with energy densities varying from 3 to 5 J/cm(2) over a period of 1-6 days. The light source was a 120 mW Ga-As diode laser (lambda = 904 nm). Growth curves and procollagen immunoprecipitation were obtained.

RESULTS:

Irradiation of 3 and 4 J/cm(2) increased the cell numbers about threefold to sixfold comparing to control cultures. However, this effect was restricted to a small range of energy densities since 5 J/cm(2) had no effect on cell growth. The energy density of 3 J/cm(2) remarkably increased cell growth, with no effect on procollagen synthesis, as demonstrated by the immunoprecipitation analysis.

CONCLUSIONS:

Our results showed that a particular laser irradiation stimulates fibroblast proliferation, without impairing procollagen synthesis.

• Authors: Aymann Nassif Pereira, Carlos de Paula Eduardo, Edmir Matson, Márcia Martins Marques
• Journal: Lasers in surgery and medicine (Lasers Surg Med) Vol. 31 Issue 4 Pg. 263-7 ( 2002) ISSN: 0196-8092 [Print] United States
• PMID: 12355572 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2002 Wiley-Liss, Inc.

Chemical References

• Procollagen

Topics

• 3T3 Cells (radiation effects)
• Animals
• Cell Division (radiation effects)
• Cells, Cultured (radiation effects)
• Fibroblasts (radiation effects)
• In Vitro Techniques
• Low-Level Light Therapy
• Mice
• Procollagen (chemical synthesis)
• Time Factors
• Wound Healing (radiation effects)