Abstract | PURPOSE: METHODS: Three types of HPMA copolymers containing the saccharide epitopes galactosamine (P-Gal), lactose (P-Lac), or triantennary galactose (P-TriGal) were synthesized. The relationship between the content of the saccharide moieties, the valency of the galactose residues, and their biorecognition by the cell lines (Colo-205, SW-480, and SW-620) was investigated using flow cytometry and confocal fluorescence microscopy analysis. RESULTS: The binding of the glycoconjugates to the human colonadenocarcinoma cell lines was dependent on the type and the number of bound sugar residues per macromolecule. The higher the sugar contents in the HPMA copolymers, the higher the extent of binding. Although introduction of galactoside residues into the HPMA copolymer resulted in a significant increase in the binding of the copolymers to the cells, low biorecognition of the lactoside-containing HPMA copolymers by all cell lines used was observed. The trivalent galactoside-containing HPMA copolymers did not yield a notable glycoside cluster effect for the beta-galactoside-binding lectin expressed on human colon-adenocarcinoma cells. Among the various cell line little differences in the extent of binding of the glycopolymers to the cells were observed. The data on the internalization of HPMA copolymer conjugates obtained by confocal fluorescence microscopy correlated well with the flow cytometry analysis of their biorecognition by target cells. CONCLUSIONS:
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Authors | Ayelet David, Pavla Kopecková, Jindrich Kopecek, Abraham Rubinstein |
Journal | Pharmaceutical research
(Pharm Res)
Vol. 19
Issue 8
Pg. 1114-22
(Aug 2002)
ISSN: 0724-8741 [Print] United States |
PMID | 12240936
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Methacrylates
- Lactose
- hydroxypropyl methacrylate
- Galactose
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Topics |
- Adenocarcinoma
(drug therapy, metabolism)
- Colonic Neoplasms
(drug therapy, metabolism)
- Galactose
(administration & dosage, chemistry, metabolism)
- Humans
- Lactose
(administration & dosage, chemistry, metabolism)
- Methacrylates
(administration & dosage, chemistry, metabolism)
- Tumor Cells, Cultured
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