Treatment of uterine leiomyosarcoma in a xenograft nude mouse model using high-intensity focused ultrasound: a potential treatment modality for recurrent pelvic disease.

Abstract

OBJECTIVE:

The objective was to test the efficacy of high-intensity focused ultrasound (HIFU) for treatment of uterine leiomyosarcoma in a Xenograft nude mouse model.

METHODS:

A total of 65 athymic nude mice were inoculated subcutaneously with 5 to 7 x 10(6) ELT-5B cells, a uterine leiomyosarcoma cell line derived from the Eker rat. Thirty animals showed tumor growth. The tumor volume was measured transcutaneously once a week. Animals were randomly assigned to three groups: HIFU treatment (n = 17), sham treatment (n = 7), and control (n = 6). A HIFU device, operating at a frequency of 2.0 MHz and an intensity of 2000 W/cm(2), was used for treatment.

RESULTS:

Within 3 weeks of a single HIFU treatment, 100% reduction in tumor volume was observed in all animals, except one. A second HIFU treatment was applied to that animal, resulting in 100% reduction in tumor volume. The tumors in the sham-treated animals continued to grow at a similar rate to that of the control group to approximately 500% of the tumor volume at the time of treatment. All animals were monitored for a maximum of 3 months. No metastasis was observed in the HIFU-treated animals. Histological examination confirmed a complete tumor disappearance after HIFU treatment.

CONCLUSION:

We have shown that HIFU can effectively treat uterine leiomyosarcoma tumors inoculated in Xenograft nude mice, demonstrating HIFU's potential use for treatment of recurrent uterine leiomyosarcoma.

• Authors: Amid Keshavarzi, Shahram Vaezy, Misty L Noble, Emil Y Chi, Cheryl Walker, Roy W Martin, Victor Y Fujimoto
• Journal: Gynecologic oncology (Gynecol Oncol) Vol. 86 Issue 3 Pg. 344-50 (Sep 2002) ISSN: 0090-8258 [Print] United States
• PMID: 12217758 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Topics

• Animals
• Female
• Leiomyosarcoma (therapy)
• Mice
• Mice, Nude
• Neoplasm Recurrence, Local (therapy)
• Rats
• Ultrasonic Therapy (methods)
• Uterine Neoplasms (therapy)
• Xenograft Model Antitumor Assays