Abstract |
Wilms tumor gene product WT1 and proteinase 3 are overexpressed antigens in acute myeloid leukemia (AML), against which cytotoxic T lymphocytes can be elicited in vitro and in murine models. We performed this study to investigate whether WT1- and proteinase 3-specific CD8 T cells spontaneously occur in AML patients. T cells recognizing HLA-A2.1-binding epitopes from WT1 or proteinase 3 could be detected ex vivo in 5 of 15 HLA-A2-positive AML patients by interferon-gamma (IFN-gamma) ELISPOT assay and flow cytometry for intracellular IFN-gamma and in 3 additional patients by flow cytometry only. T cells producing IFN-gamma in response to proteinase 3 were further characterized in one patient by 4-color flow cytometry, identifying them as CD3(+)CD8(+)CD45RA(+) CCR7(-) T cells, resembling cytotoxic effector T cells. In line with this phenotype, most of the WT1- and proteinase-reactive T cells were granzyme B(+). These results provide for the first time evidence for spontaneous T-cell reactivity against defined antigens in AML patients. These data therefore support the immunogenicity of WT1 and proteinase 3 in acute leukemia patients and the potential usefulness of these antigens for leukemia vaccines.
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Authors | Carmen Scheibenbogen, Anne Letsch, Eckhard Thiel, Alexander Schmittel, Volker Mailaender, Steffi Baerwolf, Dirk Nagorsen, Ulrich Keilholz |
Journal | Blood
(Blood)
Vol. 100
Issue 6
Pg. 2132-7
(Sep 15 2002)
ISSN: 0006-4971 [Print] United States |
PMID | 12200377
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Antigens, Neoplasm
- HLA-A2 Antigen
- WT1 Proteins
- Interferon-gamma
- GZMB protein, human
- Granzymes
- Serine Endopeptidases
- Myeloblastin
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Topics |
- Acute Disease
- Antigens, Neoplasm
(immunology)
- CD8-Positive T-Lymphocytes
(immunology)
- Case-Control Studies
- Granzymes
- HLA-A2 Antigen
(immunology)
- Humans
- Immunophenotyping
- Interferon-gamma
(analysis)
- Leukemia, Myeloid
(immunology)
- Myeloblastin
- Serine Endopeptidases
(analysis, immunology)
- WT1 Proteins
(immunology)
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