D-RNAi (messenger RNA-antisense DNA interference) as a novel defense system against cancer and viral infections.

Abstract

D-RNAi (Messenger RNA-antisense DNA interference), a novel posttranscriptional phenomenon of silencing gene expression by transfection of mRNA-aDNA hybrids, was originally observed in the effects of bcl-2 on phorbol ester-induced apoptosis in human prostate cancer LNCaP cells. This phenomenon was also demonstrated in chicken embryos and a human CD4(+) T cell line, H9. The in vivo transduction of beta-catenin D-RNAi was shown to knock out more than 99% endogenous beta-catenin gene expression, while the in cell transfection of HIV-1 D-RNAi homolog rejected viral gene replication completely. D-RNAi was found to have long-term gene knockout effects resulting from a posttranscriptional gene silencing mechanism that may involve the homologous recombination between intracellular mRNA and the mRNA components of a D-RNAi construct. These findings provide a potential intracellular defense system against cancer and viral infections.

• Authors: S L Lin, S Sukasweang, C M Chuong, S Rasheed, S Y Ying
• Journal: Current cancer drug targets (Curr Cancer Drug Targets) Vol. 1 Issue 3 Pg. 241-7 (Nov 2001) ISSN: 1568-0096 [Print] Netherlands
• PMID: 12188882 (Publication Type: Journal Article, Review)

Chemical References

• DNA, Antisense
• DNA, Complementary
• RNA, Messenger
• RNA, Small Interfering
• RNA, Untranslated

Topics

• DNA, Antisense (pharmacology)
• DNA, Complementary (genetics, metabolism)
• Gene Silencing
• Humans
• Neoplasms (genetics, therapy)
• RNA Processing, Post-Transcriptional (genetics, physiology)
• RNA, Messenger (genetics, metabolism)
• RNA, Small Interfering
• RNA, Untranslated (genetics, metabolism)
• Transcription, Genetic