Implantation of VX2 carcinoma into the liver of rabbits: a comparison of three direct-injection methods.

Abstract

The efficacy of agarose in preventing VX2 carcinoma cell leakage was evaluated and the results were compared with two traditional methods. Forty-five rabbits were divided into 3 groups: Group 1, VX2 tumor cells were injected directly into the liver and no special procedure after removal of the needle; Group 2, the puncture site was gently compressed, using an alcoholic cotton gauze, for three minutes; Group 3, 0.2 ml of heated liquid agarose was injected to seal the aperture after injection of VX2 cells. The leakage rates were 80%, 53.3% and 6.6% for group 1, group 2 and group 3, respectively. We consider agarose is a useful material in preventing the leakage in the establishment of VX2 liver tumor models.

• Authors: Wan-Yu Lin, Jeonhor Chen, Yungchang Lin, Kunwang Han
• Journal: The Journal of veterinary medical science (J Vet Med Sci) Vol. 64 Issue 7 Pg. 649-52 (Jul 2002) ISSN: 0916-7250 [Print] Japan
• PMID: 12185325 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Sepharose

Topics

• Animals
• Carcinoma (pathology)
• Disease Models, Animal
• Hot Temperature
• Injections
• Liver Neoplasms, Experimental (pathology)
• Neoplasm Seeding
• Neoplasm Transplantation (methods)
• Rabbits
• Sepharose