Abstract | BACKGROUND: OBJECTIVE: METHODS: Human normal and keloid dermal fibroblasts were propagated in a serum-free in vitro model with exposure to 0, 5, 10, or 20 microm triamcinolone acetonide for 0, 24, 72, or 96 hours. Cell counts were determined by phase contrast microscopy. Levels of bFGF and TGF-beta1 in the supernatants were determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: In our study, 20 microm triamcinolone acetonide caused statistically significant increases in the peak levels of bFGF for normal and keloid fibroblast cell lines (P < 0.05). It also caused statistically significant decreases in the level of TGF-beta1 for normal and keloid fibroblast cell lines. For the keloid fibroblasts, 10 microm triamcinolone acetonide also caused a statistically significant decrease in the level of TGF-beta1. CONCLUSION:
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Authors | Lisa A Carroll, Matthew M Hanasono, Anthony A Mikulec, Magdalena Kita, R James Koch |
Journal | Dermatologic surgery : official publication for American Society for Dermatologic Surgery [et al.]
(Dermatol Surg)
Vol. 28
Issue 8
Pg. 704-9
(Aug 2002)
ISSN: 1076-0512 [Print] United States |
PMID | 12174062
(Publication Type: Journal Article)
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Chemical References |
- Glucocorticoids
- Transforming Growth Factor beta
- Fibroblast Growth Factor 2
- Triamcinolone
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Topics |
- Analysis of Variance
- Cells, Cultured
- Enzyme-Linked Immunosorbent Assay
- Fibroblast Growth Factor 2
(biosynthesis)
- Fibroblasts
(drug effects)
- Glucocorticoids
(pharmacology)
- Humans
- Keloid
(pathology)
- Skin
(cytology)
- Transforming Growth Factor beta
(biosynthesis)
- Triamcinolone
(pharmacology)
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