Specific IgE determination to Ani s 1, a major allergen from Anisakis simplex, is a useful tool for diagnosis.

Abstract

BACKGROUND:

The most serious limitation of the serodiagnosis of parasitoses is the occurrence of cross-reactions.

OBJECTIVE:

The possible use of Anisakis simplex major allergen Ani s 1 for diagnosis.

PATIENTS AND METHODS:

Forty-nine non-fish-allergic patients with Anisakis simplex hypersensitivity, 21 patients without allergic episodes suffering intestinal anisakiasis with obstruction of the intestinal lumen, and 10 unrelated sera as a control were included in this study to determine specific immunoglobulin (Ig)E and IgG to Anisakis simplex major allergen Ani s 1 by immunoblotting.

RESULTS:

Eighty-six percent of patients with Anisakis simplex hypersensitivity showed specific IgE directed to Ani s 1. Identical result was obtained for IgG detection in this group. Among patients with intestinal anisakiasis, 86% showed specific IgE, but only 29% had specific IgG (P < 0.001, two-tailed Fisher exact test). One of the 10 control subjects was positive both for IgE and IgG (P < 0.001).

CONCLUSIONS:

Determination of specific IgE directed to Anisakis simplex major allergen Ani s 1 is a useful tool for the diagnosis of hypersensitivity and intestinal anisakiasis. Further, measurement of specific IgG directed to Anisakis simplex major allergen Ani s 1 is only valid for Anisakis simplex allergy.

• Authors: María Luisa Caballero, Ignacio Moneo
• Journal: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology (Ann Allergy Asthma Immunol) Vol. 89 Issue 1 Pg. 74-7 (Jul 2002) ISSN: 1081-1206 [Print] United States
• PMID: 12141725 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Allergens
• Calcium-Binding Proteins
• Helminth Proteins
• Immunoglobulin G
• troponin-like protein, Anisakis simplex
• Immunoglobulin E

Topics

• Allergens
• Animals
• Anisakiasis (diagnosis)
• Anisakis (immunology)
• Calcium-Binding Proteins (immunology)
• Fishes (parasitology)
• Helminth Proteins (immunology)
• Humans
• Immunoglobulin E (blood)
• Immunoglobulin G (blood)