Abstract | BACKGROUND: Inflammatory processes that occur before, during, and after surgery may contribute to damage of transplanted hearts and their ability to withstand acute and chronic rejection. METHODS: We determined the expression of mRNA for 10 inflammatory indicator molecules in hearts from brain-dead animals in which stable circulation was maintained. To produce brain death in male rats (n = 11), we inflated an intracranial balloon with saline (245 microl +/- 27 microl) to produce apnea and areflexia. Mean arterial pressure was maintained at 80 +/- 2 mm Hg for 6 hours. Controls (n = 11) received a burr hole but no balloon (mean arterial pressure, 94 +/- 1 mm Hg). We measured expression of each indicator molecule mRNA relative to expression of glyceraldehyde-3-phosphate dehydrogenase mRNA using reverse-transcriptase polymerase chain reaction. RESULTS: CONCLUSION:
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Authors | Leigh D Segel, Derek W vonHaag, Jie Zhang, David M Follette |
Journal | The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation
(J Heart Lung Transplant)
Vol. 21
Issue 7
Pg. 804-11
(Jul 2002)
ISSN: 1053-2498 [Print] United States |
PMID | 12100907
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Cell Adhesion Molecules
- Inflammation Mediators
- Interleukins
- RNA, Messenger
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Topics |
- Animals
- Brain Death
(metabolism)
- Cell Adhesion Molecules
(metabolism)
- Graft Rejection
(etiology)
- Heart Transplantation
- Inflammation Mediators
(metabolism)
- Interleukins
(metabolism)
- Male
- Models, Animal
- Myocardium
(metabolism)
- RNA, Messenger
(analysis)
- Rats
- Rats, Sprague-Dawley
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