Abstract | BACKGROUND: OBJECTIVES: To describe polymerase chain reaction (PCR) and serological methods for the detection of herpesvirus-induced CNS infections by the example of HSE. STUDY DESIGN: 620 cerebrospinal fluid (CSF) and 2400 serum samples from 2700 selected hospitalized patients with clinical suspicion of encephalitis were tested for herpes simplex virus (HSV) as well as varicella-zoster virus (VZV) DNA and HSV-specific antibodies, respectively. RESULTS: CONCLUSIONS: The detection of viral DNA by PCR technique has become the "gold standard" method for laboratory diagnosis of herpesvirus infections of CNS. Serodiagnosis may be useful to confirm the diagnosis retrospectively.
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Authors | A Sauerbrei, P Wutzler |
Journal | Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
(J Clin Virol)
Vol. 25 Suppl 1
Pg. S45-51
(Jul 2002)
ISSN: 1386-6532 [Print] Netherlands |
PMID | 12091081
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- Antibodies, Viral
- DNA, Viral
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Topics |
- Adolescent
- Adult
- Aged
- Antibodies, Viral
(blood)
- Central Nervous System Viral Diseases
(blood, cerebrospinal fluid, diagnosis)
- Child
- Child, Preschool
- DNA, Viral
(analysis)
- Encephalitis, Herpes Simplex
(blood, cerebrospinal fluid, diagnosis)
- Herpesviridae
(genetics, isolation & purification)
- Herpesviridae Infections
(blood, cerebrospinal fluid, diagnosis)
- Herpesvirus 3, Human
(genetics, isolation & purification)
- Humans
- Infant
- Infant, Newborn
- Middle Aged
- Polymerase Chain Reaction
- Simplexvirus
(genetics, isolation & purification)
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