Abstract | BACKGROUND: Inhibition of Na(+)/H(+) exchange has been shown to provide functional protection during ischemia and reperfusion in mature heart. This study was undertaken to elucidate the effect of Na(+)/H(+) exchange inhibitor HOE642 in the aged rabbit heart. METHODS: Isolated rabbit hearts were subjected to 1 h of left descending coronary artery (LAD) ischemia and 1 h of reperfusion. To determine the effects of HOE642 on ischemia/reperfusion injury, seven aged or mature hearts received the Na(+)/H(+) exchange inhibitor HOE642 (1 microM) for 15 min before the ischemia and for 30 min after reperfusion. Seven aged (more than 135 weeks) or mature (15-20 weeks) rabbit hearts served as a control (untreated) with no interventions. Left ventricular pressures, monophasic action potentials and coronary flows were measured throughout the experiment and infarct size was detected at the end of experiment. RESULTS: (1) In the mature hearts, HOE642 improved postischemic functional recovery (63.1 +/- 5.0% vs. 84.4 +/- 5.4%, mature untreated vs. mature HOE, p < 0.05) and reduced infarct size as compared to untreated hearts (42.0 +/- 2.5% vs. 24.8 +/- 2.3%, mature untreated vs. mature HOE, p < 0.05). (2) Although infarct size in aged untreated hearts was significantly decreased as compared to mature untreated hearts (42.0 +/- 2.5% vs. 19.3 +/- 1.6%, mature untreated vs. aged untreated, p < 0.05), there are no significant differences regarding postischemic functional recovery between mature and aged untreated hearts (63.1 +/- 5.0% vs. 59.5 +/- 5.9%, mature untreated vs. aged untreated, p = n.s.). (3) In the aged hearts, HOE642 improved postischemic functional recovery as compared to untreated hearts (59.5 +/- 5.9% vs. 85.9 +/- 8.1%, aged untreated vs. aged HOE, p < 0.05). CONCLUSION:
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Authors | Yasunari Nakai, H Horimoto, S Mieno, S Sasaki |
Journal | European surgical research. Europaische chirurgische Forschung. Recherches chirurgicales europeennes
(Eur Surg Res)
2002 May-Jun
Vol. 34
Issue 3
Pg. 244-50
ISSN: 0014-312X [Print] Switzerland |
PMID | 12077512
(Publication Type: Journal Article)
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Copyright | Copyright 2002 S. Karger AG, Basel |
Chemical References |
- Guanidines
- Sodium-Hydrogen Exchangers
- Sulfones
- cariporide
|
Topics |
- Action Potentials
(drug effects)
- Aging
(metabolism)
- Animals
- Coronary Circulation
(drug effects)
- Guanidines
(pharmacology)
- Heart
(drug effects, physiopathology)
- In Vitro Techniques
- Ischemic Preconditioning, Myocardial
- Male
- Models, Cardiovascular
- Myocardial Infarction
(pathology, prevention & control)
- Myocardial Reperfusion Injury
(physiopathology, prevention & control)
- Myocardium
(metabolism)
- Rabbits
- Sodium-Hydrogen Exchangers
(antagonists & inhibitors)
- Sulfones
(pharmacology)
- Ventricular Function, Left
(drug effects)
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