Abstract |
Osteoarthritis has as main characteristics the degradation of articular cartilage and the formation of new bone at the joint edges, so-called osteophytes. In this study enhanced expression of TGF-beta1 and -beta3 was detected in developing osteophytes and articular cartilage during murine experimental osteoarthritis. To determine the role of endogenous TGF-beta on osteophyte formation and articular cartilage, TGF-beta activity was blocked via a scavenging soluble TGF-beta-RII. Our results clearly show that inhibition of endogenous TGF-beta nearly completely prevented osteophyte formation. In contrast, treatment with recombinant soluble TGF-beta-RII markedly enhanced articular cartilage proteoglycan loss and reduced the thickness of articular cartilage. In conclusion, we show for the first time that endogenous TGF-beta is a crucial factor in the process of osteophyte formation and has an important function in protection against cartilage loss.
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Authors | Alwin Scharstuhl, Harrie L Glansbeek, Henk M van Beuningen, Elly L Vitters, Peter M van der Kraan, Wim B van den Berg |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 169
Issue 1
Pg. 507-14
(Jul 01 2002)
ISSN: 0022-1767 [Print] United States |
PMID | 12077282
(Publication Type: Journal Article)
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Chemical References |
- Protein Isoforms
- RNA, Messenger
- Receptors, Transforming Growth Factor beta
- Recombinant Proteins
- Tgfb1 protein, mouse
- Tgfb3 protein, mouse
- Tissue Inhibitor of Metalloproteinases
- Transforming Growth Factor beta
- Transforming Growth Factor beta1
- Transforming Growth Factor beta3
- Protein Serine-Threonine Kinases
- Receptor, Transforming Growth Factor-beta Type II
- ADAM Proteins
- ADAMTS5 Protein
- Adamts5 protein, mouse
- Collagenases
- Matrix Metalloproteinase 13
- Metalloendopeptidases
- Mmp13 protein, mouse
- Procollagen N-Endopeptidase
- Matrix Metalloproteinase 1
- ADAMTS4 Protein
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Topics |
- ADAM Proteins
- ADAMTS4 Protein
- ADAMTS5 Protein
- Animals
- Arthritis, Experimental
(immunology, metabolism, pathology)
- Blotting, Western
- Cartilage, Articular
(chemistry, drug effects, immunology, pathology)
- Cell Differentiation
(drug effects, immunology)
- Chondrocytes
(drug effects, immunology, pathology)
- Collagenases
(biosynthesis, genetics)
- Electrophoresis, Polyacrylamide Gel
- Immunohistochemistry
- Male
- Matrix Metalloproteinase 1
(biosynthesis, genetics)
- Matrix Metalloproteinase 13
- Metalloendopeptidases
(biosynthesis, genetics)
- Mice
- Mice, Inbred C57BL
- Osteoarthritis, Knee
(immunology, metabolism, pathology)
- Osteogenesis
(drug effects, immunology)
- Pichia
(enzymology)
- Procollagen N-Endopeptidase
- Protein Isoforms
(analysis, antagonists & inhibitors, biosynthesis)
- Protein Serine-Threonine Kinases
- RNA, Messenger
(biosynthesis)
- Receptor, Transforming Growth Factor-beta Type II
- Receptors, Transforming Growth Factor beta
(analysis, biosynthesis, physiology)
- Recombinant Proteins
(analysis, biosynthesis, pharmacology)
- Solubility
- Tissue Inhibitor of Metalloproteinases
(biosynthesis, genetics)
- Transforming Growth Factor beta
(analysis, antagonists & inhibitors, biosynthesis)
- Transforming Growth Factor beta1
- Transforming Growth Factor beta3
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