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Newly established clear cell sarcoma (malignant melanoma of soft parts) cell line expressing melanoma-associated Melan-A antigen and overexpressing C-MYC oncogene.

Abstract
Clear cell sarcoma (CCS), malignant melanoma of soft parts, is a rare malignant tumor with a poor prognosis. In this study, a CCS cell line, designated MP-CCS-SY, was established from a metastatic tumor of a 17-year-old Japanese girl that originated in the left Achilles tendon. A small number of melanosomes were detected in the cytoplasm by electron microscopy. The melanosomes immunoreacted with two melanoma-associated antibodies, HMB45 and Melan-A. A Western blot demonstrated the existence of a Melan-A antigen in this cell line. Although a t(12;22)(q13;q12), which is characteristic of CCS, was not identified by a chromosomal analysis with conventional banding techniques, fluorescence in situ hybridization analysis with painting probes of chromosomes 12 and 22 revealed the insertion of a chromosome 12 fragment into one of the long arms of chromosome 22. The chimeric EWS/ATF1 transcript was detected by the reverse transcriptase polymerase chain reaction. Extra copies and structural abnormalities of chromosome 8 were observed. Overexpression of c-myc mRNA was detected by Northern blot analysis and may have a role in malignant progression of CCS. The availability of this MP-CCS-SY cell line will help to understand the molecular biology of this malignancy and should be useful as a tool for developing an immunotherapy.
AuthorsHiroshi Moritake, Tohru Sugimoto, Yujiro Asada, Mitsuaki A Yoshida, Yoshihiko Maehara, Alan L Epstein, Hiroshi Kuroda
JournalCancer genetics and cytogenetics (Cancer Genet Cytogenet) Vol. 135 Issue 1 Pg. 48-56 (May 2002) ISSN: 0165-4608 [Print] United States
PMID12072203 (Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antigens, Neoplasm
  • EWS-ATF1 fusion protein, human
  • MART-1 Antigen
  • MLANA protein, human
  • Neoplasm Proteins
  • Oncogene Proteins, Fusion
  • Proto-Oncogene Proteins c-myc
  • RNA, Messenger
  • RNA, Neoplasm
  • Transcription Factors
Topics
  • Achilles Tendon (pathology)
  • Adolescent
  • Antigens, Neoplasm
  • Chromosomes, Human, Pair 12 (genetics, ultrastructure)
  • Chromosomes, Human, Pair 22 (genetics, ultrastructure)
  • Chromosomes, Human, Pair 8 (genetics, ultrastructure)
  • Disease Progression
  • Fatal Outcome
  • Female
  • Gene Expression Regulation, Neoplastic
  • Genes, myc
  • Humans
  • In Situ Hybridization, Fluorescence
  • MART-1 Antigen
  • Melanosomes (chemistry, ultrastructure)
  • Mutagenesis, Insertional
  • Neoplasm Proteins (biosynthesis, genetics)
  • Oncogene Proteins, Fusion (genetics)
  • Proto-Oncogene Proteins c-myc (biosynthesis)
  • RNA, Messenger (biosynthesis, genetics)
  • RNA, Neoplasm (biosynthesis, genetics)
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sarcoma, Clear Cell (genetics, metabolism, pathology)
  • Soft Tissue Neoplasms (genetics, metabolism, pathology)
  • Transcription Factors
  • Tumor Cells, Cultured (metabolism)

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