Abstract |
The aim of the study was the evaluation of angiogenesis processes in fibrosarcoma induced by 3-methylcholanthrene (3-Mc) in reference to the number of mast cells (MCs). 76 male Wistar rats were divided into 4 groups: two experimental (E) groups--after injection of 0.2 mg 3-Mc dissolved in olive oil (0.25ml), and 2 control (C) groups. In E1 group, 52 rats were killed after development of the fibrosarcoma; E(2)--10 rats were killed before development of the tumor; C(1)-8 rats received 0.25ml olive oil; C(2)--8 rats received no treatment. Tissue material was fixed in buffered formalin or Carnoy's and Bouin's fluid. Paraffin sections were stained with H+E and Azan methods, and with alcian blue-saphranine and toluidine blue. Immunohistochemical reactions detecting tryptase in MCs were also performed. Angiogenic objects (microvessels and single endothelial cells) were recognized using antibodies against factor VIII (vWF), P selectin (CD-62P), and CD-90. We found a distinct relationship between intensification of neoangiogenesis at the tumor periphery and increased number of MCs.
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Authors | B Sawicki, I Kasacka, L Chyczewski |
Journal | Folia histochemica et cytobiologica
(Folia Histochem Cytobiol)
Vol. 40
Issue 2
Pg. 197-8
( 2002)
ISSN: 0239-8508 [Print] Poland |
PMID | 12056643
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers, Tumor
- Carcinogens
- Methylcholanthrene
- Serine Endopeptidases
- Tpsab1 protein, rat
- Tpsb2 protein, rat
- Tryptases
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Topics |
- Animals
- Biomarkers, Tumor
(analysis)
- Carcinogens
- Fibrosarcoma
(blood supply, chemically induced, pathology)
- Immunohistochemistry
- Male
- Mast Cells
(pathology)
- Methylcholanthrene
- Neovascularization, Pathologic
(pathology)
- Rats
- Rats, Wistar
- Regional Blood Flow
(physiology)
- Serine Endopeptidases
(metabolism)
- Staining and Labeling
- Tissue Embedding
- Tryptases
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