Expression of p53 and p21 Ki-ras proteins in rat thyroid gland tumors induced by 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX).

Abstract

BACKGROUND:

3-Chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone (MX), a disinfection by-product in chlorinated drinking water, induces follicular tumors in thyroid glands in Wistar rats. The mechanisms of the MX-induced thyroid gland tumorigenesis are not known.

MATERIALS AND METHODS:

The expressions of p53 (primary antibody CM5) and p21 Ki-ras (primary antibody F234) proteins were evaluated by immunohistochemisty in MX-induced tumors in Wistar rats. p53 expression was studied in 3 follicular adenomas, 29 follicular carcinomas and two C-cell carcinomas of thyroid glands. p21 Ki-ras expression was studied in 13 follicular carcinomas and one C-cell carcinoma.

RESULTS:

A weak expression of p53 protein (1-5% of tumor cells) observed in six follicular carcinomas (21%) and one C-cell carcinoma was not considered to be p53-positive. No expression of p21 Ki-ras was observed in any of the samples.

CONCLUSION:

These data indicate that p53 and Ki-ras proteins are not overexpressed in the MX-induced thyroid tumors in rats.

• Authors: Pasi Hakulinen, Veli-Matti Kosma, Hannu Komulainen
• Journal: Anticancer research (Anticancer Res) 2002 Mar-Apr Vol. 22 Issue 2A Pg. 703-6 ISSN: 0250-7005 [Print] Greece
• PMID: 12014640 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Carcinogens
• Furans
• Tumor Suppressor Protein p53
• 3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone
• Proto-Oncogene Proteins p21(ras)

Topics

• Animals
• Carcinogens (toxicity)
• Furans (toxicity)
• Proto-Oncogene Proteins p21(ras) (biosynthesis)
• Rats
• Rats, Wistar
• Thyroid Neoplasms (chemically induced, metabolism)
• Tumor Suppressor Protein p53 (biosynthesis)