Abstract | OBJECTIVE: MATERIALS AND METHODS: Male rats had their pudendal arteries ligated or underwent a sham operation. They were then treated by an intracavernosal injection with 4 microg of VEGF in phosphate-buffered saline (PBS) or PBS alone. At 6 and 24 h after treatment electrostimulation was applied to the cavernosal nerve and the intracorporal pressure measured. The erectile tissue was then harvested for RNA isolation and cryo-sectioning. The isolated RNA was used for microarray and reverse transcription-polymerase chain reaction (RT-PCR) analyses, and the tissue sections for immunohistochemical analysis. RESULTS: Microarray analysis detected nNOS, eNOS and iNOS at very low expression levels in PBS-treated rats; expression levels were higher for eNOS and iNOS in all VEGF-treated rats. These results were further confirmed by RT-PCR analysis. Immunohistochemical analysis identified the cavernosal endothelium and smooth muscle as the tissue types where eNOS and iNOS were up-regulated, respectively. CONCLUSIONS: This is the first report of the induction of both eNOS and iNOS in the penis after intracavernosal VEGF. These events may help support a significant recovery of erectile function after interrupting the blood supply to the penis.
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Authors | C-S Lin, H-C Ho, K-C Chen, G Lin, L Nunes, T F Lue |
Journal | BJU international
(BJU Int)
Vol. 89
Issue 9
Pg. 955-60
(Jun 2002)
ISSN: 1464-4096 [Print] England |
PMID | 12010247
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Carrier Proteins
- Endothelial Growth Factors
- RNA, Messenger
- Vascular Endothelial Growth Factor A
- Nitric Oxide Synthase
- Nitric Oxide Synthase Type II
- Nos2 protein, rat
- NOSIP protein, human
- Ubiquitin-Protein Ligases
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Topics |
- Animals
- Carrier Proteins
(genetics, metabolism)
- Endothelial Growth Factors
(administration & dosage, pharmacology)
- Immunohistochemistry
(methods)
- Impotence, Vasculogenic
(enzymology)
- Injections
- Male
- Nitric Oxide Synthase
(genetics, metabolism)
- Nitric Oxide Synthase Type II
- Penis
(drug effects)
- RNA, Messenger
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Reverse Transcriptase Polymerase Chain Reaction
- Ubiquitin-Protein Ligases
- Vascular Endothelial Growth Factor A
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