The effect of
castration on apoptosis in the mouse epididymis during postnatal development was examined. The weight of the epididymis slowly increased from day 0 (day of birth) to day 20 after birth, followed by a rapid increase thereafter.
Castration on days 0, 5, 10, 20, 30, 40 and 60 increased apoptotic indices (percentages of apoptotic cells) of epithelia of the caput (head), corpus (body), and cauda (tail) epididymis, their apoptotic indices reaching maximal levels on day 2 after
castration with the exception of a maximal apoptotic index on day 4 in the tail after
castration on day 60. The maximal levels of apoptotic indices of the head, body and tail after
castration on days 0, 5, 10 and 20 were significantly lower than those after
castration on days 40 and 60. DNAs extracted from the epididymides 2 days after
castration on days 0, 5, 10 and 60 showed a ladder pattern on
agarose gel electrophoresis, which is a characteristic of apoptosis. When
testosterone propionate (10 microg/g
body weight) was injected twice a day into mice which had been castrated on day 10, 30 or 60, the increases in apoptotic indices of the head, body and tail of the epididymis were completely inhibited. The weights of the paired epididymides 6 days after
castration on days 0, 5, 10, 20, 30, 40 and 60 were significantly lower than those of
sham-operated mice, indicating the secretion of
androgen by the testes from birth to adulthood. The present results indicated that
androgen deprivation caused by
castration induces apoptosis in the epithelium of the epididymis of mice from birth to adulthood, and suggested that a proportion of epithelial cells, the survival of which is dependent on the testes, is smaller in the epididymides during a slow growth stage than in the epididymides after this stage.