IgG anti-IgE autoantibodies in visceral leishmaniasis.

Abstract

Procedures for IgG depletion in visceral leishmaniasis (VL) and schistosomiasis sera using Sepharose-protein G beads also deplete IgE. In this study, the presence of IgG anti-IgE autoantibodies in sera from patients with VL (n = 10), and hepatic-intestinal schistosomiasis (n = 10) and from healthy individuals (n = 10) was investigated. A sandwich ELISA using goat IgG anti-human IgE to capture serum IgE and goat anti-human IgG peroxidase conjugate to demonstrate the binding of IgG to the IgE captured was performed. VL sera had higher titers (p < 0.05) of IgG anti-IgE autoantibodies (OD = 2.01 +/- 0.43) than sera from healthy individuals (OD = 1.35 +/- 0.16) or persons infected with Schistosoma mansoni (OD = 1.34 +/- 0.18). The immunoblotting carried out with eluates from Sepharose-protein G beads used to deplete IgG from these sera and goat anti-human IgE peroxidase conjugate, showed a similar pattern of bands, predominating the 75 kDa epsilon-heavy chain and also polypeptides resulting from physiological enzymatic digestion of IgE. A frequent additional band immediately above 75 kDa was observed only in VL sera.

• Authors: A M Atta, M L B Sousa-Atta, A D'Oliveira, R P Almeida, M I Araújo, E M Carvalho
• Journal: Memorias do Instituto Oswaldo Cruz (Mem Inst Oswaldo Cruz) Vol. 97 Issue 1 Pg. 101-3 (Jan 2002) ISSN: 0074-0276 [Print] Brazil
• PMID: 11992157 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Anti-Idiotypic
• Autoantibodies
• Immunoglobulin G
• Immunoglobulin E
• Sepharose
• GTP-Binding Proteins

Topics

• Antibodies, Anti-Idiotypic (blood, immunology)
• Autoantibodies (blood, immunology)
• GTP-Binding Proteins (metabolism)
• Humans
• Immunoglobulin E (blood, immunology)
• Immunoglobulin G (blood, immunology)
• Leishmaniasis, Visceral (blood, immunology)
• Protein Binding
• Schistosomiasis mansoni (blood, immunology)
• Sepharose (metabolism)