Cross-linked matrix tissue sealant protects against mortality and hemorrhage in an acute renal injury model in heparinized rats.

Abstract

PURPOSE:

We evaluated the efficacy of a cross-linked matrix tissue sealant compared with oxidized cellulose and electrocautery in an acute renal injury model.

MATERIALS AND METHODS:

Sprague-Dawley rats underwent partial nephrectomy after intravenous heparin anticoagulation. The cut surface received 1 of 4 therapies, namely no treatment, cross-linked matrix tissue sealant, electrocautery or Surgicel (Johnson & Johnson, New Brunswick, New Jersey) oxidized cellulose. Blood pressure was continually monitored, survival time was determined and total blood loss was quantified in each animal.

RESULTS:

Animals that received no treatment, electrocautery or oxidized cellulose died within 13 minutes of injury with a decline in blood pressure to 0 mm. Hg. In cross-linked matrix treated rats hemostasis was immediate, resulting in no blood loss after cross-linked matrix application. Mean arterial pressure +/- SEM was maintained at 136 +/- 6 mm. Hg. All cross-linked matrix treated animals survived.

CONCLUSIONS:

Cross-linked matrix tissue sealant provided immediate hemostasis and protection from a decline in blood pressure after renal trauma in heparinized rats. Cross-linked matrix tissue sealant may benefit patients with severe renal injuries. In the future cross-linked matrix may be useful for long-term partial nephrectomy repair.

• Authors: Peter B Petratos, Keiichi Ito, Diane Felsen, Dix Poppas
• Journal: The Journal of urology (J Urol) Vol. 167 Issue 5 Pg. 2222-4 (May 2002) ISSN: 0022-5347 [Print] United States
• PMID: 11956482 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Cellulose, Oxidized
• Cross-Linking Reagents
• Hemostatics
• Surgicel

Topics

• Animals
• Cellulose, Oxidized
• Cross-Linking Reagents (pharmacology)
• Electrocoagulation
• Hemorrhage (mortality, pathology)
• Hemostatics (pharmacology)
• Kidney (injuries, pathology)
• Male
• Rats
• Rats, Sprague-Dawley
• Wounds, Penetrating (mortality, pathology)