HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

[Detection of cytomegalovirus infection with the polymerase chain reaction and antigenemia after allogenic bone marrow transplantation].

AbstractUNLABELLED:
The objective of the work was to evaluate the frequency and time of incidence of cytomegaloviral (CMV) infection and disease in patients after allogeneic bone marrow transplantation (BMT). One hundred patients were followed up (70 with a related and 30 with an unrelated donor), who had transplantations during the period between XI/1996-XI/2000.
METHODS USED:
nested-PCR (MIE-gene) and antigenaemia (antigen pp65). Active CMV infection was proved in antigenaemia > or = 5 positive cells or in two consecutive positive PCR. The CMV syndrome was assessed in confirmed CMV infection and otherwise inexplicable febrile conditions and/or a drop of haemogram values. For the diagnosis of CMV pneumonia the clinical picture was needed, evidence of active CMV infection and on the X-ray of the lungs interstitial pneumonia. In 33 patients both methods were used, in 67 only PCR. The first positive test appeared 6-321 days after BMT (median +/- 49 days). CMV infection was proved in 44% cases, CMV syndrome in 30% and CMV pneumonia in 4%. In patients with a related donor CMV infection was found in 34.3%, CMV syndrome in 22.9%, CMV pneumonia in 1.4%. After unrelated donor BMT CMV infection was recorded in 66.7%, CMV syndrome in 46.7% and CMV pneumonia in 10% patients. Two patients died from CMV pneumonia. CMV pneumonia was diagnosed 57-115 days after BMT (median +/- 68 days. The risk of CMV infection is high in both groups of patients, in particular in patients after unrelated donor BMT (66.7%). As far as the development of CMV pneumonia was concerned, the mortality in the authors' group was 50%.
AuthorsP Simková, P Jindra, V Koza, K Cerná
JournalVnitrni lekarstvi (Vnitr Lek) Vol. 48 Issue 2 Pg. 120-4 (Feb 2002) ISSN: 0042-773X [Print] Czech Republic
Vernacular TitleSledování výskytu cytomegalovirové infekce u nemocných po alogenní transplantaci kostní drenÄ• pomocí polymerázové retÄ•zové reakce (PCR) a antigenémie.
PMID11949219 (Publication Type: English Abstract, Journal Article)
Chemical References
  • Antigens, Viral
  • DNA, Viral
  • Immunoglobulin G
Topics
  • Adult
  • Antigens, Viral (blood)
  • Bone Marrow Transplantation (adverse effects)
  • Cytomegalovirus (genetics, immunology)
  • Cytomegalovirus Infections (diagnosis, etiology)
  • DNA, Viral (blood)
  • Family
  • Female
  • Humans
  • Immunoglobulin G (blood)
  • Living Donors
  • Male
  • Middle Aged
  • Polymerase Chain Reaction

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: