Nested PCR for diagnosis of tuberculous lymphadenitis and PCR-SSCP for identification of rifampicin resistance in fine-needle aspirates.

Abstract

An accurate diagnosis of tuberculosis and multidrug resistance is important for the control of tuberculosis, which remains a major public health problem. Fine-needle aspiration (FNA) has provided an alternative tool for bacterial examination. This study was performed to investigate the usefulness of one-step polymerase chain reaction (PCR) and PCR-SSCP as a routine test for the detection of Mycobacterium tuberculosis and rifampicin-resistant strain in FNA. Ziehl-Neelsen stain (Z-N) and PCR were processed using the aspirates of tuberculous lymphadenitis for the detection of M. tuberculosis. PCR-SSCP was done for the identification of rpoB mutation. M. tuberculosis was detected in 49/63 (77.8%) by PCR and 25/63 (39.7%) by Z-N. There were 26 cases with PCR(+)/Z-N(-) and two cases with PCR(-)/Z-N(+). Twelve cases showed negativity against both. In 7/22 (31.8%), rpoB mutation was observed. In conclusion, PCR is more sensitive in the detection of M. tuberculosis in FNA than Z-N. PCR-SSCP could also be used in FNA in the prediction of multidrug resistance.

• Authors: Gyungyub Gong, Hungkyung Lee, Gyeong Hoon Kang, Yhoung-hee Shim, Jooryung Huh, Shin Kwang Khang
• Journal: Diagnostic cytopathology (Diagn Cytopathol) Vol. 26 Issue 4 Pg. 228-31 (Apr 2002) ISSN: 8755-1039 [Print] United States
• PMID: 11933268 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2002 Wiley-Liss, Inc.

Chemical References

• Antibiotics, Antitubercular
• DNA, Bacterial
• Rifampin

Topics

• Antibiotics, Antitubercular (pharmacology)
• Biopsy, Needle (methods)
• DNA, Bacterial (analysis)
• Drug Resistance, Microbial (genetics)
• Humans
• Lymph Nodes (microbiology, pathology)
• Lymphadenitis (microbiology, pathology)
• Mycobacterium tuberculosis (drug effects, genetics, isolation & purification)
• Polymerase Chain Reaction
• Polymorphism, Single-Stranded Conformational
• Rifampin (pharmacology)
• Tuberculosis, Lymph Node (complications, pathology)