Clonality studies in giant cell tumor of bone.

Abstract

Genetic studies including chromosome analysis, telomere reduction and telomere activity, DNA microsatellites and loss of heterozygosity (LOH) studies have been performed on giant cell tumor (GCT) of bone however whether this primary skeletal neoplasm represents a monoclonal or polyclonal proliferation is unknown. Utilizing a new assay to study the polymorphic human androgen receptor locus (HUMARA), the ratio of maternal inactive X-chromosome to the paternal inactive X (Lyon hypothesis) is determined via a methylation--specific polymerase chain reaction (PCR) technique to detect X-chromosome polymorphisms. Characterization of the genetic tumorigenesis of this unpredictable neoplasm may lend insight into its biological behavior and offer improvements in therapeutic intervention, as new information emerges regarding osteoclastic bone resorption. Seventeen female patients with giant cell tumor of bone had their DNA harvested and their X-chromosome inactivation pattern and polymorphisms determined and compared to control. A polyclonal proliferation pattern was identified in all informative samples studied.

• Authors: H S Schwartz, J D Eskew, M G Butler
• Journal: Journal of orthopaedic research : official publication of the Orthopaedic Research Society (J Orthop Res) Vol. 20 Issue 2 Pg. 387-90 (Mar 2002) ISSN: 0736-0266 [Print] United States
• PMID: 11918322 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• AR protein, human
• DNA, Neoplasm
• Receptors, Androgen

Topics

• Adult
• Bone Neoplasms (genetics, pathology)
• Child
• Clone Cells
• DNA, Neoplasm (analysis)
• Female
• Giant Cell Tumor of Bone (genetics, pathology)
• Heterozygote
• Humans
• Middle Aged
• Polymerase Chain Reaction
• Polymorphism, Genetic
• Receptors, Androgen
• X Chromosome