Abstract | OBJECTIVES: DESIGN: A lymphoblast model was used, as this has previously been used in the study of hypertension and of NADPH oxidase. Chemiluminescence (CL) was chosen to assay ROS production, as it is simple and sensitive. METHODS: Lymphocytes from 12 hypertensive patients (HT), and 12 age- and sex-matched normotensive (NT) subjects, were immortalized. Luminol, isoluminol and Cypridina luciferin analogue (CLA) CL were used to assay ROS production. NADPH oxidase subunits were measured by Western blot analysis. RESULTS: CONCLUSIONS: We have shown there is increased ROS production in lymphoblasts derived from hypertensive subjects, probably originating from NADPH oxidase. As the ROS production persists in transformed cells, this suggests a genetic predisposition to increased ROS production. Increased expression of p22( phox) in HT lymphoblasts may account for some of the increased ROS.
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Authors | Andrew I Pettit, Richard K M Wong, Virginia Lee, Sonja Jennings, Pauline A Quinn, Leong L Ng |
Journal | Journal of hypertension
(J Hypertens)
Vol. 20
Issue 4
Pg. 677-83
(Apr 2002)
ISSN: 0263-6352 [Print] England |
PMID | 11910303
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Free Radicals
- Membrane Transport Proteins
- Phosphoproteins
- Arachidonic Acid
- NADPH Oxidases
- CYBA protein, human
- NADPH Dehydrogenase
- Tetradecanoylphorbol Acetate
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Topics |
- Arachidonic Acid
(pharmacology)
- Cell Line
- Female
- Free Radicals
(metabolism)
- Humans
- Hypertension
(enzymology, metabolism)
- Luminescent Measurements
- Lymphocytes
(drug effects, metabolism)
- Male
- Membrane Transport Proteins
- Middle Aged
- NADPH Dehydrogenase
(metabolism)
- NADPH Oxidases
- Phosphoproteins
(metabolism)
- Tetradecanoylphorbol Acetate
(pharmacology)
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