Abstract | BACKGROUND: RESULTS:
SB 203580 had a dichotomal effect in TNBS mice. Weight loss of TNBS mice treated with SB 203580 was significantly worse and colon weight on sacrifice was significantly increased in MAPK inhibitor treated TNBS mice (229.2 mg and 289.1 mg, respectively). However, the total number of cells in the caudal lymph node decreased to 188.8 x 10(4) cells in SB 203580 treated TNBS mice compared with 334 x 10(4) cells in vehicle treated mice. CD3/CD28 double stimulated caudal lymph node cells of SB 203580 treated mice showed decreased interferon gamma production but increased tumour necrosis factor alpha production. The concentration of interleukin 12p70 in colon homogenates was significantly decreased in SB 203580 treated mice whereas concentrations of interleukin 12p40, tumour necrosis factor alpha, and interleukin 10 were similar in vehicle and SB 203580 treated TNBS mice. CONCLUSION: Our results reveal a dichotomy in p38 MAPK action during experimental colitis.
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Authors | T ten Hove, B van den Blink, I Pronk, P Drillenburg, M P Peppelenbosch, S J H van Deventer |
Journal | Gut
(Gut)
Vol. 50
Issue 4
Pg. 507-12
(Apr 2002)
ISSN: 0017-5749 [Print] England |
PMID | 11889071
(Publication Type: Journal Article)
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Chemical References |
- Enzyme Inhibitors
- Imidazoles
- Pyridines
- Tumor Necrosis Factor-alpha
- Interferon-gamma
- Trinitrobenzenesulfonic Acid
- Mitogen-Activated Protein Kinases
- p38 Mitogen-Activated Protein Kinases
- SB 203580
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Topics |
- Animals
- Blotting, Western
- Colitis
(chemically induced, enzymology)
- Enzyme Activation
- Enzyme Inhibitors
(pharmacology)
- Enzyme-Linked Immunosorbent Assay
- Imidazoles
(pharmacology)
- Interferon-gamma
(metabolism)
- Mice
- Mice, Inbred BALB C
- Mitogen-Activated Protein Kinases
(antagonists & inhibitors, metabolism)
- Pyridines
(pharmacology)
- T-Lymphocytes
(metabolism)
- Trinitrobenzenesulfonic Acid
- Tumor Necrosis Factor-alpha
(metabolism)
- Wasting Syndrome
(etiology)
- Weight Loss
- p38 Mitogen-Activated Protein Kinases
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