Abstract |
We previously showed that an intraperitoneal infection with mouse hepatitis virus (MHV) persists in interferon-gamma (IFN-gamma)-deficient C57BL/6 (B6-GKO) mice and results in subacute fatal peritonitis, which bears a resemblance to feline infectious peritonitis. To examine the role of other host factors in MHV infection in mice, IFN-gamma-deficient mice with a BALB/c background (BALB-GKO) were infected intraperitoneally with MHV and compared with B6-GKO mice. In contrast to B6-GKO mice, BALB-GKO mice died within 1 week due to acute hepatic failure. The viral titer of the liver in BALB-GKO mice was significantly higher than that in B6-GKO mice. All hepatocytes in BALB-GKO mice were necrotic at 5 days post- infection, which was clearly distinct from large but limited lesion in the liver from infected B6-GKO mice. The serum alanine aminotransferase activity of infected BALB-GKO mice were higher than that of B6-GKO mice and was paralleled with the severity of the pathological changes and viral titers in infected mice. Administration of exogenous IFN-gamma to BALB-GKO partially inhibited the acute death. These results indicate that BALB-GKO and B6-GKO mice clearly show different diseases following MHV infection, although wild type counterparts of both mice apparently showed the same clinical course after MHV infection.
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Authors | Shigeru Kyuwa, Shinwa Shibata, Yoh-ichi Tagawa, Yoichiroh Iwakura, Kenji Machii, Toru Urano |
Journal | Virus research
(Virus Res)
Vol. 83
Issue 1-2
Pg. 169-77
(Feb 26 2002)
ISSN: 0168-1702 [Print] Netherlands |
PMID | 11864749
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Interferon-gamma
- Alanine Transaminase
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Topics |
- Alanine Transaminase
(blood)
- Animals
- Coronavirus Infections
(blood, immunology, pathology, virology)
- Hepatitis, Viral, Animal
(blood, immunology, pathology, virology)
- Injections, Intraperitoneal
- Interferon-gamma
(administration & dosage, genetics, immunology)
- Liver Failure, Acute
(blood, immunology, pathology, virology)
- Mice
- Mice, Inbred BALB C
- Mice, Knockout
- Murine hepatitis virus
(growth & development, immunology)
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