Glutathione S-transferase-pi in malignant tissues and plasma of human colorectal and gastric cancers.

Abstract

PURPOSE:

In this study, 16 paired samples of colorectal and gastric cancers and adjacent non-neoplastic tissues were analysed for the determination of glutathione S-transferase (GST) activities and the expression of GST-pi.

METHODS:

Western blotting procedure as well as plasma GST-pi levels were used.

RESULTS:

GST activities were found to be increased in malignant tissues of patient compared with adjacent normal tissues. A significant correlation was detected between GST activity and GST-pi expression in malignant tissues of patients. Plasma GST-pi levels increased in patients compared to aged-matched control subjects. When the patients were grouped according to TNM stage, GST-pi expression in malignant tissues as well as plasma GST-pi levels were higher in patients with more advanced tumor stages.

CONCLUSIONS:

Our results indicate that GST-pi expression in malignant tissues and plasma GST-pi levels in human colorectal and gastric cancers increased depending on the stages of tumor.

• Authors: S Doğru-Abbasoğlu, U Mutlu-Türkoğlu, S Türkoğlu, Y Erbil, U Barbaros, M Uysal, G Aykaç-Toker
• Journal: Journal of cancer research and clinical oncology (J Cancer Res Clin Oncol) Vol. 128 Issue 2 Pg. 91-5 (Feb 2002) ISSN: 0171-5216 [Print] Germany
• PMID: 11862479 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Isoenzymes
• GSTP1 protein, human
• Glutathione S-Transferase pi
• Glutathione Transferase

Topics

• Adult
• Aged
• Aged, 80 and over
• Blotting, Western
• Colorectal Neoplasms (enzymology, pathology)
• Electrophoresis, Polyacrylamide Gel
• Female
• Gene Expression Regulation, Neoplastic
• Glutathione S-Transferase pi
• Glutathione Transferase (biosynthesis, metabolism)
• Humans
• Isoenzymes (biosynthesis, metabolism)
• Male
• Middle Aged
• Neoplasm Staging
• Stomach Neoplasms (enzymology, pathology)