Abstract |
Detection of MRD remains one of the major goals in the treatment of acute lymphoblastic leukemia (ALL). We have used the polymerase chain reaction (PCR)-heteroduplex (HD) analysis to assess and confirm the clonal expansion of T cell receptor (TCR) gamma and delta gene rearrangements in 24 T-ALL patients at diagnosis. 52.4% revealed Vdelta1-Jdelta1; 48% Vdelta2-Ddelta3; 62.5% Vgamma1-Jgamma1 and 46% both Vdelta1-Jdelta1 and Vgamma1-Jgamma1 clonal rearrangements. 6/24 patients had TAL-1 deletion. These clonal markers were used to monitor MRD in remission/relapse bone marrow samples for periods ranging from 6 to 75 months after diagnosis. Patients who relapsed and died revealed a continuous PCR-HD positivity in their clinical remission bone marrow samples. HD analysis established identical diagnostic clone at relapse. Patients who are in long-term clinical and morphological remission achieved PCR-HD negativity in their 8-12 months bone marrow remission samples and continue to be PCR-HD negative. MRD monitored in six patients with two diagnostic PCR--HD positive clonal markers reveal an identical pattern ensuring circumvention of false positive and negative results. Thus, we conclude that PCR followed by HD analysis is a useful technique to monitor MRD in remission/relapse samples in ALL patients.
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Authors | K Nirmala, K R Rajalekshmy, S G Raman, V Shanta, T Rajkumar |
Journal | Leukemia research
(Leuk Res)
Vol. 26
Issue 4
Pg. 335-43
(Apr 2002)
ISSN: 0145-2126 [Print] England |
PMID | 11839375
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Basic Helix-Loop-Helix Transcription Factors
- DNA, Neoplasm
- DNA-Binding Proteins
- Proto-Oncogene Proteins
- T-Cell Acute Lymphocytic Leukemia Protein 1
- Transcription Factors
- TAL1 protein, human
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Topics |
- Adolescent
- Adult
- Basic Helix-Loop-Helix Transcription Factors
- Child
- Child, Preschool
- DNA, Neoplasm
(analysis, genetics)
- DNA-Binding Proteins
(genetics)
- Female
- Gene Deletion
- Gene Rearrangement, delta-Chain T-Cell Antigen Receptor
(genetics)
- Gene Rearrangement, gamma-Chain T-Cell Antigen Receptor
(genetics)
- Humans
- Leukemia-Lymphoma, Adult T-Cell
(diagnosis, genetics, pathology)
- Male
- Neoplasm, Residual
- Polymerase Chain Reaction
- Predictive Value of Tests
- Proto-Oncogene Proteins
- Recurrence
- T-Cell Acute Lymphocytic Leukemia Protein 1
- Transcription Factors
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