[The biological effect of verapamil on hypertrophic scar fibroblast].

Abstract

OBJECTIVE:

To study the mechanism of calcium channel antagonist Verapamil on the treatment of hypertrophic scar and explore the possibility of further clinical application.

METHODS:

After six strains of HSFB were cultured in vitro, we investigated HSFB proliferation by MTT method, investigated HSFB collagen synthesis by 3H-proline uptaken and Hydroxyproline colorimetric analysis, and investigated collagen gene expression by Northern Blot.

RESULTS:

Verapamil can inhibit HSFB proliferation, collagen synthesis and gene expression by a dose-depended manner, especially treated with 100 mumol/L Verapamil.

CONCLUSION:

By inhibiting I, III procollagen gene expression, Verapamil can inhibit the formation of hypertrophic scar.

• Authors: Z Wang, S Guo, K Lu
• Journal: Zhonghua zheng xing wai ke za zhi = Zhonghua zhengxing waike zazhi = Chinese journal of plastic surgery (Zhonghua Zheng Xing Wai Ke Za Zhi) Vol. 17 Issue 6 Pg. 328-31 (Nov 2001) ISSN: 1009-4598 [Print] China
• PMID: 11838051 (Publication Type: English Abstract, Journal Article)

Chemical References

• Calcium Channel Blockers
• Verapamil

Topics

• Adolescent
• Calcium Channel Blockers (pharmacology)
• Cell Division (drug effects)
• Cells, Cultured
• Child
• Child, Preschool
• Cicatrix, Hypertrophic (pathology)
• Female
• Fibroblasts (drug effects)
• Humans
• Male
• Verapamil (pharmacology)