Hypoxia-inducible factor 1 alpha (HIF-1 alpha) that regulates genes involved in response to
hypoxia and promotes neo-angiogenesis, is a transcriptional factor for vascular endothelial cell
growth factor (
VEGF). The aim of this study was to examine the expression of HIF-1 alpha and
VEGF gene expressions and their relation to angiogenesis, clinicopathologic variables and survival in the patient with human ovarian
carcinoma. We retrospectively analyzed HIF-1 alpha and
VEGF gene expression levels using
reverse transcriptase polymerase chain reaction (RT-PCR) in 60 ovarian
carcinomas. Intratumoral microvessel density (IMD) was assessed by immunostaining endothelial cells, using anti-CD 31 antibody in frozen sections. The relationships between the expression level of these genes, IMD and clinicopathologic variables were evaluated by Student's t-test and chi-square tests. Survival analysis was performed by Kaplan-Meier curves. HIF-1 alpha or
VEGF gene expression level was independent of age, clinical stage and histological subtype besides grade of
tumor. There was no relationship between HIF-1 alpha or
VEGF gene expression level and IMD in all
carcinomas (R=0.118 and 0.224, respectively). In addition, a weak association between HIF-1 alpha and
VEGF gene expression level was observed (R=0.300, P=0.020). The association between
VEGF gene expression and IMD was observed (R=0.501, P=0.016). However, no association between IMD and HIF-1 alpha gene expression was observed. Further, both HIF-1 alpha and
VEGF gene expression levels had no effect on survival in the patient with ovarian
carcinoma. These results suggest that
VEGF upregulated by HIF-1 alpha gene may be involved in angiogenesis of some type of ovarian
carcinoma, but the expression levels of both genes have no effect on survival in the patients with ovarian
carcinoma.