[Experimental study on human pancreatic carcinoma treated by cytosine deaminase gene therapy].

Abstract

OBJECTIVE:

To explore the possibility of Escherichia coli cytosine deaminase (CD) gene on human pancreatic carcinoma gene therapy.

METHODS:

Recombinant adenoviruses containing a carcinoembryonic antigen (CEA) promoter were transiently introduced into SW1990, Capan-2 and Hela cells, separately. The expression of CD gene mRNA was examined by RT-PCR. CD protein level in the transduced cells was analyzed by Western blotting. The sensitivity of the cells to 5-fluorocytosine (5-FC) was determined by MTT assay.

RESULTS:

A specific expression of cytosine deaminase gene by adenovirus-mediated transfer exhibited only in SW1990 cells (CEA-producing). Transduction of CD gene resulted in significant sensitivity of SW1990 cells to 5-FC. The anticancer effect was seen in vivo in SW1990 xenografts nude mice with in situ CD gene transduction.

CONCLUSION:

The targeted expression of CD gene combined prodrug 5-FC may be a potential approach for gene therapy for human pancreatic carcinoma.

• Authors: S Zhang, S Yuan
• Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi) Vol. 80 Issue 4 Pg. 249-51 (Apr 2000) ISSN: 0376-2491 [Print] China
• PMID: 11798763 (Publication Type: Journal Article)

Chemical References

• Antimetabolites, Antineoplastic
• Carcinoembryonic Antigen
• Culture Media
• Prodrugs
• Flucytosine
• Nucleoside Deaminases
• Cytosine Deaminase

Topics

• Adenocarcinoma (therapy)
• Adenoviruses, Human (genetics, physiology)
• Animals
• Antimetabolites, Antineoplastic (pharmacology)
• Carcinoembryonic Antigen (analysis, genetics)
• Culture Media
• Cytosine Deaminase
• Escherichia coli (enzymology)
• Flucytosine (pharmacology)
• Gene Expression
• Genetic Therapy (methods)
• Genetic Vectors (genetics, physiology)
• HeLa Cells
• Humans
• Mice
• Mice, Nude
• Neoplasms, Experimental (pathology, therapy)
• Nucleoside Deaminases (biosynthesis, genetics)
• Pancreatic Neoplasms (therapy)
• Prodrugs (pharmacology)
• Promoter Regions, Genetic
• Tumor Cells, Cultured