Abstract | OBJECTIVE: METHODS: Recombinant adenoviruses containing a carcinoembryonic antigen (CEA) promoter were transiently introduced into SW1990, Capan-2 and Hela cells, separately. The expression of CD gene mRNA was examined by RT-PCR. CD protein level in the transduced cells was analyzed by Western blotting. The sensitivity of the cells to 5-fluorocytosine (5-FC) was determined by MTT assay. RESULTS: A specific expression of cytosine deaminase gene by adenovirus-mediated transfer exhibited only in SW1990 cells (CEA-producing). Transduction of CD gene resulted in significant sensitivity of SW1990 cells to 5-FC. The anticancer effect was seen in vivo in SW1990 xenografts nude mice with in situ CD gene transduction. CONCLUSION:
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Authors | S Zhang, S Yuan |
Journal | Zhonghua yi xue za zhi
(Zhonghua Yi Xue Za Zhi)
Vol. 80
Issue 4
Pg. 249-51
(Apr 2000)
ISSN: 0376-2491 [Print] China |
PMID | 11798763
(Publication Type: Journal Article)
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Chemical References |
- Antimetabolites, Antineoplastic
- Carcinoembryonic Antigen
- Culture Media
- Prodrugs
- Flucytosine
- Nucleoside Deaminases
- Cytosine Deaminase
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Topics |
- Adenocarcinoma
(therapy)
- Adenoviruses, Human
(genetics, physiology)
- Animals
- Antimetabolites, Antineoplastic
(pharmacology)
- Carcinoembryonic Antigen
(analysis, genetics)
- Culture Media
- Cytosine Deaminase
- Escherichia coli
(enzymology)
- Flucytosine
(pharmacology)
- Gene Expression
- Genetic Therapy
(methods)
- Genetic Vectors
(genetics, physiology)
- HeLa Cells
- Humans
- Mice
- Mice, Nude
- Neoplasms, Experimental
(pathology, therapy)
- Nucleoside Deaminases
(biosynthesis, genetics)
- Pancreatic Neoplasms
(therapy)
- Prodrugs
(pharmacology)
- Promoter Regions, Genetic
- Tumor Cells, Cultured
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