Heat shock inhibits IL-12 p40 expression through NF-kappa B signalling pathway in murine macrophages.

Abstract

We report the effect of heat shock on lipopolysaccharide (LPS)-induced interleukin 12 (IL-12) expression. The augmentation of LPS-induced IL-12 p40 mRNA and p70 protein was significantly suppressed in both peritoneal macrophages and RAW264.7 cells after heat shock at 43 degrees C. The binding activity of nuclear factor kappa B (NF-kappa B) was reduced by prior heat shock. LPS did not induce degradation of the inhibitory protein I-kappa B alpha in the shocked cells, which might be a potential mechanism to block NF-kappa B activation. Furthermore, transient transfection assay in RAW264.7 cells demonstrated that LPS-induced activation of DM703 and DM138 (contains NF-kappa B motif) was highly sensitive to heat shock. These data suggest that heat shock influences expression of IL-12 through the I-kappa B/NF-kappa B pathway.

• Authors: C L Li, X Y Wang, J Shao, J S Zhang, W G Feng, Y B Wang, Z L Chang
• Journal: Cytokine (Cytokine) Vol. 16 Issue 4 Pg. 153-9 (Nov 21 2001) ISSN: 1043-4666 [Print] England
• PMID: 11792125 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2001 Academic Press.

Chemical References

• DNA-Binding Proteins
• I-kappa B Proteins
• Interleukin-12 Subunit p40
• Lipopolysaccharides
• NF-kappa B
• Nfkbia protein, mouse
• Protein Subunits
• RNA, Messenger
• NF-KappaB Inhibitor alpha
• Interleukin-12

Topics

• Animals
• Binding Sites (genetics)
• Cell Line
• DNA-Binding Proteins (metabolism)
• Gene Expression
• Hot Temperature
• I-kappa B Proteins
• In Vitro Techniques
• Interleukin-12 (biosynthesis, genetics)
• Interleukin-12 Subunit p40
• Lipopolysaccharides (pharmacology)
• Macrophages (drug effects, immunology, metabolism)
• Macrophages, Peritoneal (drug effects, immunology, metabolism)
• Mice
• NF-KappaB Inhibitor alpha
• NF-kappa B (metabolism)
• Promoter Regions, Genetic
• Protein Subunits
• RNA, Messenger (genetics, metabolism)
• Signal Transduction