Abstract | BACKGROUND: Gastric juice vitamin C may be protective against gastric carcinogenesis but concentrations are significantly reduced by Helicobacter pylori infection. We investigated the in vitro effects of vitamin C at concentrations comparable with those found in gastric juice on gastric cancer cells and H pylori. METHODS:
Gastric cancer cell lines and various H pylori strains were treated with L-ascorbic acid for up to 72 hours. Cell viability, and protein and DNA synthesis were determined. Flow cytometry was used for assessment of H pylori adherence, cell cycle distribution, and apoptosis. H pylori growth and its haemagglutination activity were determined using viability count and microtitration assay. RESULTS:
Vitamin C induced a significant dose dependent growth inhibition of gastric AGS and MKN45 cells but this effect was significantly reduced at levels similar to those in gastric juice of H pylori infected patients (<50 microM). Although vitamin C had no obvious effect on H pylori growth, haemagglutination activity, or adherence ability to gastric AGS cells compared with untreated controls, it significantly enhanced H pylori associated apoptosis and induced cell cycle arrest in these cells. CONCLUSION:
Vitamin C may inhibit gastric cancer cell growth and alter H pylori induced cell cycle events at concentrations comparable with those in gastric juice, but has no effect on H pylori growth or pathogenicity. However, the inhibitory effect on gastric cancer cells was lost at vitamin C concentrations found in patients with H pylori infection.
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Authors | Z-W Zhang, M Abdullahi, M J G Farthing |
Journal | Gut
(Gut)
Vol. 50
Issue 2
Pg. 165-9
(Feb 2002)
ISSN: 0017-5749 [Print] England |
PMID | 11788554
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Adenocarcinoma
(pathology, prevention & control)
- Ascorbic Acid
(physiology)
- Bacterial Adhesion
(physiology)
- Cell Division
- Flow Cytometry
(methods)
- Gastric Juice
(chemistry)
- Helicobacter Infections
(prevention & control)
- Helicobacter pylori
(growth & development, pathogenicity, physiology)
- Hemagglutination
(physiology)
- Hemagglutination Tests
- Humans
- Hydrogen-Ion Concentration
- Intestinal Mucosa
(microbiology)
- Stomach Neoplasms
(pathology, prevention & control)
- Tumor Cells, Cultured
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