HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Effect of physiological concentrations of vitamin C on gastric cancer cells and Helicobacter pylori.

AbstractBACKGROUND:
Gastric juice vitamin C may be protective against gastric carcinogenesis but concentrations are significantly reduced by Helicobacter pylori infection. We investigated the in vitro effects of vitamin C at concentrations comparable with those found in gastric juice on gastric cancer cells and H pylori.
METHODS:
Gastric cancer cell lines and various H pylori strains were treated with L-ascorbic acid for up to 72 hours. Cell viability, and protein and DNA synthesis were determined. Flow cytometry was used for assessment of H pylori adherence, cell cycle distribution, and apoptosis. H pylori growth and its haemagglutination activity were determined using viability count and microtitration assay.
RESULTS:
Vitamin C induced a significant dose dependent growth inhibition of gastric AGS and MKN45 cells but this effect was significantly reduced at levels similar to those in gastric juice of H pylori infected patients (<50 microM). Although vitamin C had no obvious effect on H pylori growth, haemagglutination activity, or adherence ability to gastric AGS cells compared with untreated controls, it significantly enhanced H pylori associated apoptosis and induced cell cycle arrest in these cells.
CONCLUSION:
Vitamin C may inhibit gastric cancer cell growth and alter H pylori induced cell cycle events at concentrations comparable with those in gastric juice, but has no effect on H pylori growth or pathogenicity. However, the inhibitory effect on gastric cancer cells was lost at vitamin C concentrations found in patients with H pylori infection.
AuthorsZ-W Zhang, M Abdullahi, M J G Farthing
JournalGut (Gut) Vol. 50 Issue 2 Pg. 165-9 (Feb 2002) ISSN: 0017-5749 [Print] England
PMID11788554 (Publication Type: Journal Article)
Chemical References
  • Ascorbic Acid
Topics
  • Adenocarcinoma (pathology, prevention & control)
  • Ascorbic Acid (physiology)
  • Bacterial Adhesion (physiology)
  • Cell Division
  • Flow Cytometry (methods)
  • Gastric Juice (chemistry)
  • Helicobacter Infections (prevention & control)
  • Helicobacter pylori (growth & development, pathogenicity, physiology)
  • Hemagglutination (physiology)
  • Hemagglutination Tests
  • Humans
  • Hydrogen-Ion Concentration
  • Intestinal Mucosa (microbiology)
  • Stomach Neoplasms (pathology, prevention & control)
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: