Effects of platelet activating factor antagonist (BN50739) on gut mucosal injury in acute severe pancreatitis in pigs.

Abstract

OBJECTIVE:

To study the role of platelet activating factor (PAF) in the pathogenesis of intestinal mucosal injury and endotoxin/bacterial translocation in acute severe pancreatitis (ASP) in pigs.

METHODS:

ASP was induced by intraductal injection of a mixture of sodium taurocholate and trypsin. BN50739, a specific antagonist of PAF, was given 30 min prior to the induction of ASP. Mucosal blood flow, mucosal myeloperoxidase (MPO) and malondialdehyde (MDA) were determined. Intestinal injury was observed microscopically. Portal blood endotoxin levels and the bacterial counts in the portal blood, intestinal lymph nodes and the pancreas were determined.

RESULTS:

Prior antagonism of PAF by BN50739 reduced intestinal injury, increased intestinal mucosal blood flow, and reduced blood levels of endotoxin and bacterial counts in the portal blood, mesenteric lymph nodes and pancreas.

CONCLUSIONS:

Intestinal mucosal injury developed in ASP. PAF is responsible for the injury. Antagonism of PAF by BN50739 can improve intestinal microcirculation and reduce the severity of intestinal mucosal injury, which may decrease endotoxin/bacterial translocation.

• Authors: W Zhu, J Li, W Tu, N Li
• Journal: Chinese medical journal (Chin Med J (Engl)) Vol. 113 Issue 8 Pg. 756-8 (Aug 2000) ISSN: 0366-6999 [Print] China
• PMID: 11776064 (Publication Type: Journal Article)

Chemical References

• Azepines
• Platelet Activating Factor
• Triazoles
• BN 50739

Topics

• Acute Disease
• Animals
• Azepines (pharmacology)
• Intestinal Mucosa (drug effects, pathology)
• Pancreatitis (chemically induced, pathology)
• Platelet Activating Factor (antagonists & inhibitors)
• Swine
• Triazoles (pharmacology)