Comparison of serum and urinary C-terminal telopeptide of type I collagen in aging, menopause and osteoporosis.

Abstract

BACKGROUND:

Urinary C-terminal telopeptide of type I collagen (u-CTx) has been reported to be a sensitive biochemical marker of bone turnover. There have been two assays for urinary CTx, which are alpha-CTx and beta-CTx. A newly developed immunoassay for serum CTx (s-CTx) is now available for assessment of bone resorption. We evaluated the effects of aging, menopause, and osteoporosis on the measurements of serum CTx and compared them to urinary CTx assays.

METHODS:

In 79 premenopausal healthy women, 80 postmenopausal healthy women, 61 osteoporotic patients with vertebral fractures and 34 osteoporotic patients with hip fractures, s-CTx and urinary beta-CTx (u-betaCTx) were measured by ELISAs, and urinary alpha-CTx (u-alphaCTx) was measured by an RIA.

RESULTS:

In all subjects, s-CTx significantly correlated with both u-alphaCTx (r=0.54) and u-betaCTx (r=0.51). There was no significant difference among s-CTx, u-alphaCTx and u-betaCTx in the T-scores of the postmenopausal group over the premenopausal group. These findings indicate that the value of s-CTx, as well as urinary CTxs, reflected the increase of bone resorption associated with menopause with a high degree of sensitivity. Patients with vertebral fractures had moderately increased concentrations of bone resorption markers compared to age-matched healthy postmenopausal women (T-score; s-CTx: 0.8, u-alphaCTx: 0.9, u-betaCTx: 0.7), whereas bone resorption markers in hip fracture patients were greatly increased compared to healthy postmenopausal women (T-score; s-CTx: 1.1, u-alphaCTx: 1.3 u-betaCTx: 1.3). The T-scores of u-CTxs against the postmenopausal group in vertebral fracture group and in hip fracture group were not significantly different from those of s-CTx.

CONCLUSIONS:

s-CTx, as well as urinary CTxs, reflects the increase of bone resorption in patients with vertebral fractures and hip fractures.

• Authors: K Kawana, M Takahashi, H Hoshino, K Kushida
• Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta) Vol. 316 Issue 1-2 Pg. 109-15 (Feb 2002) ISSN: 0009-8981 [Print] Netherlands
• PMID: 11750280 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Biomarkers
• Collagen Type I
• Peptides
• Reagent Kits, Diagnostic
• collagen type I trimeric cross-linked peptide
• Collagen

Topics

• Adult
• Aged
• Aged, 80 and over
• Aging (blood, pathology, urine)
• Biomarkers (blood, urine)
• Bone Resorption (blood, urine)
• Case-Control Studies
• Collagen (blood, urine)
• Collagen Type I
• Enzyme-Linked Immunosorbent Assay (standards)
• Female
• Hip Fractures (blood, urine)
• Humans
• Menopause (blood, urine)
• Middle Aged
• Osteoporosis, Postmenopausal (blood, urine)
• Peptides (blood, urine)
• Radioimmunoassay (standards)
• Reagent Kits, Diagnostic (standards)
• Spinal Fractures (blood, urine)