Abstract | AIM: METHODS: The expression of Bcl-2, Bcl-X(L), and Mcl-1 was investigated in normal biliary epithelium (17), biliary dysplasia (three), and invasive cholangiocarcinoma (51), in addition to three human cholangiocarcinoma cell lines, by immunohistochemistry and immunofluorescence. RESULTS: The expression of Bcl-2 was not detected in normal or malignant biliary tissue. In contrast, granular cytoplasmic Bcl-X(L) and Mcl-1 staining was found in 60-100% of cells in all normal, dysplastic, and malignant specimens, including the human cell lines examined in this study. CONCLUSION: These findings indicate that Mcl-1 and Bcl-X(L), but not Bcl-2, are involved in the survival of normal and neoplastic cells in the biliary tree. By prolonging survival through blocking apoptosis, these proteins might be reducing the efficacy of cytotoxic anticancer treatments in cholangiocarcinoma.
|
Authors | A C Okaro, A R Deery, R R Hutchins, B R Davidson |
Journal | Journal of clinical pathology
(J Clin Pathol)
Vol. 54
Issue 12
Pg. 927-32
(Dec 2001)
ISSN: 0021-9746 [Print] England |
PMID | 11729212
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- BCL2L1 protein, human
- Myeloid Cell Leukemia Sequence 1 Protein
- Neoplasm Proteins
- Proto-Oncogene Proteins c-bcl-2
- bcl-X Protein
|
Topics |
- Apoptosis
(physiology)
- Bile Duct Neoplasms
(chemistry)
- Bile Ducts
(chemistry)
- Cell Line
- Cholangiocarcinoma
(chemistry)
- Epithelial Cells
(chemistry)
- Humans
- Image Processing, Computer-Assisted
- Immunohistochemistry
- Microscopy, Fluorescence
- Myeloid Cell Leukemia Sequence 1 Protein
- Neoplasm Proteins
(analysis)
- Precancerous Conditions
(chemistry)
- Proto-Oncogene Proteins c-bcl-2
(analysis)
- bcl-X Protein
|