The objective of this study was to examine whether a decrease in neutrophil-mediated tissue injury using
Fucoidin, a nontoxic neutrophil rolling inhibitor, would improve flap survival in an
island flap model after
ischemia-reperfusion.
Myeloperoxidase activity (an indirect index of tissue neutrophil count) and
malondialdehyde (an
indicator of lipid peroxidation), the degree of neutrophil infiltration by direct counting, and macroscopic flap survival were assessed in the flap after arterial
ischemia-reperfusion. Epigastric island skin flaps were elevated in 56 rats. The first group of 21 rats was subjected to 6 hours of arterial
ischemia. The second group of 21 rats was subjected to 10 hours of arterial
ischemia, and the rest of the rats were used as nonischemic controls (
sham flaps). For inhibiting neutrophil rolling, a nontoxic
polysaccharide agent-
Fucoidin-was used. Each ischemic group was divided further into three subgroups: Subgroup I (control rats) received saline, subgroup II received 10 mg per kilogram
Fucoidin, and subgroup III received 25 mg per kilogram
Fucoidin before reperfusion. The results were evaluated as tissue neutrophil counts, tissue
malondialdehyde content, tissue
myeloperoxidase activity, and flap survival. Neutrophil counts and tissue
myeloperoxidase activity were decreased significantly (p <0.001) in subgroup III, but lipid peroxidation by means of tissue
malondialdehyde content was not affected by
Fucoidin administration. The authors conclude that administration of
Fucoidin before reperfusion can limit tissue injury apparently by inhibiting neutrophil rolling in a dose-dependent manner.