Abstract | OBJECTIVE: Investigation of the expression of sterol regulatory element binding protein-1c (SREBP-1c) in different adipose tissue depots in morbidly obese subjects before and after 1 year of weight loss induced by gastric banding operation. RESEARCH METHODS AND PROCEDURES:
SREBP-1c expression was studied in 20 massively obese subjects (6 men and 14 women; age: 41 +/- 9 years; weight: 148 +/- 34 kg; percentage of body fat: 42 +/- 4; mean +/- SD) using reverse transcription competitive polymerase chain reaction. Adipose tissue biopsies were taken from omental, subcutaneous abdominal, and femoral depots before weight loss, and from subcutaneous depots after weight loss. Subcutaneous samples were taken also from 6 normal weight subjects. RESULTS: The level of SREBP-1c mRNA was significantly lower in omental (1.8 +/- 0.2 amol/microg of total RNA) than in subcutaneous abdominal (3.7 +/- 0.4 amol/microg of total RNA) or femoral (3.9 +/- 0.4 amol/microg of total RNA; p < 0.001, mean +/- SEM) depots. The values in subcutaneous depots were about twice as high in normal weight (7.4 +/- 2.5 for abdominal and 6.5 +/- 1.5 for femoral, p < 0.01) as in obese subjects. After weight loss, the mRNA levels of SREBP-1c increased in obese subjects, both in subcutaneous abdominal (5.3 +/- 0.7, p < 0.01) and in femoral (4.8 +/- 0.8, p < 0.05) tissue. DISCUSSION:
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Authors | M Kolehmainen, H Vidal, E Alhava, M I Uusitupa |
Journal | Obesity research
(Obes Res)
Vol. 9
Issue 11
Pg. 706-12
(Nov 2001)
ISSN: 1071-7323 [Print] United States |
PMID | 11707537
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- CCAAT-Enhancer-Binding Proteins
- DNA-Binding Proteins
- RNA, Messenger
- SREBF1 protein, human
- Sterol Regulatory Element Binding Protein 1
- Transcription Factors
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Topics |
- Abdomen
- Adipose Tissue
(chemistry, pathology)
- Adult
- Anthropometry
- Biopsy
- CCAAT-Enhancer-Binding Proteins
(genetics)
- DNA-Binding Proteins
(genetics)
- Female
- Femur
- Gastroplasty
- Gene Expression
- Glucose Tolerance Test
- Humans
- Male
- Middle Aged
- Obesity, Morbid
(genetics, surgery)
- Omentum
- RNA, Messenger
(analysis)
- Reverse Transcriptase Polymerase Chain Reaction
- Skin
- Sterol Regulatory Element Binding Protein 1
- Tissue Distribution
- Transcription Factors
- Weight Loss
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