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CD80(B7.1) and CD86(B7.2) do not have distinct roles in setting the Th1/Th2 balance in autoimmunity in rats.

Abstract
Some data suggest that the interaction between CD28 and CD80 (B7.1) stimulates Th1-responses and that CD28 and CD86 (B7.2) stimulates Th2-responses, however this is controversial. We addressed this issue by using mercuric chloride (HgCl2)-induced autoimmunity in Brown Norway (BN) rats as a highly polarized Th2 model and experimental autoimmune encephalomyelitis (EAE) in Lewis rats as a highly polarized Th1 model. Monoclonal antibodies (MoAbs) to CD80 and CD86, given singly, had little effect in either model, however when given together they almost completely suppressed the HgCl2-induced autoimmunity: the peak immunoglobulin (Ig)E concentration was 3.25 microg/ml in treated animals versus 2770 microg/ml in controls (P < 0.0001); caecal vasculitis was suppressed with a median vasculitis score of 0 in treated animals versus 6 in controls (P < 0.0001); and new germinal centre formation was significantly suppressed. A combination of the antibodies also markedly reduced the severity of clinical EAE; from a median aggregate clinical score of 9 to 3 (P = 0.02) and delayed the onset from a median of 12.5 days to 16 days after immunization (P = 0.006). We have demonstrated profound suppression of both Th1 and Th2-driven autoimmunity in rats by a combination of anti-CD80 and CD86, but have been unable to demonstrate any clear differential effects.
AuthorsI A MacPhee, D R Turner, H Yagita, D B Oliveira
JournalScandinavian journal of immunology (Scand J Immunol) Vol. 54 Issue 5 Pg. 486-94 (Nov 2001) ISSN: 0300-9475 [Print] England
PMID11696200 (Publication Type: Journal Article)
Chemical References
  • Antibodies, Monoclonal
  • Antigens, CD
  • B7-1 Antigen
  • B7-2 Antigen
  • Cd86 protein, rat
  • Membrane Glycoproteins
  • Immunoglobulin E
  • Mercuric Chloride
Topics
  • Animals
  • Antibodies, Monoclonal (pharmacology)
  • Antigens, CD (metabolism)
  • Autoimmunity (drug effects)
  • B7-1 Antigen (metabolism)
  • B7-2 Antigen
  • Encephalomyelitis, Autoimmune, Experimental (immunology)
  • Female
  • Immunoglobulin E (biosynthesis)
  • Lymphocyte Activation
  • Male
  • Membrane Glycoproteins (metabolism)
  • Mercuric Chloride (toxicity)
  • Rats
  • Rats, Inbred BN
  • Rats, Inbred Lew
  • Th1 Cells (immunology)
  • Th2 Cells (immunology)
  • Vasculitis (immunology)
  • Weight Loss (immunology)

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