Chimeric papillomavirus-like particles expressing a foreign epitope on capsid surface loops.

Abstract

Neutralization capsid epitopes are important determinants for antibody-mediated immune protection against papillomavirus (PV) infection and induced disease. Chimeric L1 major capsid proteins of the human PV type 16 (HPV-16) and the bovine PV type 1 (BPV-1) with a foreign peptide incorporated into several capsid surface loops self-assembled into pentamers or virus-like particles (VLP). Binding patterns of neutralizing monoclonal antibodies (MAb) and immunization of mice confirmed (i) that regions around aa 282-286 and 351-355 contribute to neutralization epitopes and identified the latter region as an immunodominant site and (ii) that placing a foreign peptide in the context of an assembled structure markedly enhanced its immunogenicity. Pentamers disassembled from wild-type HPV-16 and BPV-1 VLPs displayed some of the neutralization epitopes that were detected on fully assembled VLPs, but were deficient for binding a subset of neutralizing MAb that inhibit cell attachment.

• Authors: Katharina Slupetzky, Saeed Shafti-Keramat, Petra Lenz, Sabine Brandt, Andreas Grassauer, Margit Sara, Reinhard Kirnbauer
• Journal: The Journal of general virology (J Gen Virol) Vol. 82 Issue Pt 11 Pg. 2799-2804 (Nov 2001) ISSN: 0022-1317 [Print] England
• PMID: 11602792 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Monoclonal
• Antibodies, Viral
• Immunodominant Epitopes
• Recombinant Fusion Proteins
• Viral Vaccines

Topics

• Animals
• Antibodies, Monoclonal (metabolism)
• Antibodies, Viral (blood)
• Bovine papillomavirus 1 (genetics, immunology, metabolism)
• Capsid (genetics, immunology, metabolism)
• Cattle
• Humans
• Immunization
• Immunodominant Epitopes
• Mice
• Mice, Inbred BALB C
• Neutralization Tests
• Papillomaviridae (genetics, immunology, metabolism)
• Papillomavirus Infections (prevention & control)
• Recombinant Fusion Proteins (genetics, immunology, metabolism)
• Tumor Virus Infections (prevention & control)
• Viral Vaccines (immunology)
• Virion (genetics, immunology)