Abstract |
The objective of this study was to assess the pharmacokinetics and bioavailability of 3beta-hydroxy-17-(1H-1,2,3-triazol-1-yl)androsta-5,16-diene ( VN/87-1) in normal male mice and in SCID mice bearing human LNCaP tumor xenografts. VN/87-1 is a novel potent steroidal inhibitor of human testicular 17-alpha-hydroxylase/C( 17,20)-lyase. The steroid also shows anti-androgenic activity and inhibits the growth of human prostate cancer cell lines (LNCaP) in vitro and in vivo. Male Balb/c mice were given a single oral, subcutaneous (s.c.) or intravenous (i.v.) bolus dose of VN/87-1 (25, 50 or 100 mg/kg). Male SCID mice bearing LNCaP tumor xenografts were injected with a single s.c. dose of VN/87-1 (50 mg/kg). The animals were sacrificed at various times up to 24 h after drug administration and blood was collected. The plasma samples were prepared and analyzed by a reversed phase HPLC system equipped with a diode array detector. A non-compartmental pharmacokinetic approach was used to evaluate the plasma level versus time data. Following i.v. administration of VN/87-1, the plasma levels declined exponentially with an elimination half-life of 1.2+/-0.03 h. The absolute bioavailability of the 50 mg/kg dose after oral or s.c. administration was 12.08+/-2 or 57.2+/-4.5%, respectively. VN/87-1 is a high clearance (5.0+/-1.3 l/h per kg) compound in mice and its volume of distribution was relatively large (6.5+/-1.2 l/kg). The pharmacokinetic parameters of VN/87-1 were not significantly altered in SCID mice bearing human LNCaP tumor xenografts. VN/87-1 is well absorbed from the subcutaneous site compared with absorption from the gastrointestinal tract and shows linear kinetics at doses up to 100 mg/kg.
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Authors | I P Nnane, V C Njar, A A Brodie |
Journal | The Journal of steroid biochemistry and molecular biology
(J Steroid Biochem Mol Biol)
Vol. 78
Issue 3
Pg. 241-6
(Sep 2001)
ISSN: 0960-0760 [Print] England |
PMID | 11595504
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- 3-hydroxy-17-(1H-1,2,3-triazol-1-yl)androsta-5,16-diene
- Androgen Antagonists
- Androstanols
- Triazoles
- Steroid 17-alpha-Hydroxylase
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Topics |
- Androgen Antagonists
(pharmacokinetics, pharmacology)
- Androstanols
(pharmacokinetics, pharmacology)
- Animals
- Biological Availability
- Humans
- Male
- Mice
- Mice, Inbred BALB C
- Mice, SCID
- Neoplasm Transplantation
- Prostatic Neoplasms
(drug therapy, metabolism)
- Steroid 17-alpha-Hydroxylase
(antagonists & inhibitors)
- Transplantation, Heterologous
- Triazoles
(pharmacokinetics, pharmacology)
- Tumor Cells, Cultured
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