HOMEPRODUCTSSERVICESCOMPANYCONTACTFAQResearchDictionaryPharmaMobileSign Up FREE or Login

Fumarylacetoacetate, the metabolite accumulating in hereditary tyrosinemia, activates the ERK pathway and induces mitotic abnormalities and genomic instability.

Abstract
Patients suffering from the metabolic disease hereditary tyrosinemia type I (HT1), caused by fumarylacetoacetate hydrolase deficiency, have a high risk of developing liver cancer. We report that a sub-apoptogenic dose of fumarylacetoacetate (FAA), the mutagenic metabolite accumulating in HT1, induces spindle disturbances and segregational defects in both rodent and human cells. Mitotic abnormalities, such as distorted spindles, lagging chromosomes, anaphase/telophase chromatin bridges, aberrant karyokinesis/cytokinesis and multinucleation were observed. Some mitotic asters displayed a large pericentriolar material cloud and/or altered distribution of the spindle pole-associated protein NuMA. FAA-treated cells developed micronuclei which were predominantly CREST-positive, suggesting chromosomal instability. The Golgi complex was rapidly disrupted by FAA, without evident microtubules/tubulin alterations, and a sustained activation of the extracellular signal-regulated protein kinase (ERK) was also observed. Primary skin fibroblasts derived from HT1 patients, not exogenously treated with FAA, showed similar mitotic-derived alterations and ERK activation. Biochemical data suggest that FAA causes ERK activation through a thiol-regulated and tyrosine kinase-dependent, but growth factor receptor- and protein kinase C-independent pathway. Pre-treatment with the MEK inhibitor PD98059 and the Ras farnesylation inhibitor B581 decreased the formation of CREST-positive micronuclei by approximately 75%, confirming the partial contribution of the Ras/ERK effector pathway to the induction of chromosomal instability by FAA. Replenishment of intracellular glutathione (GSH) with GSH monoethylester abolished ERK activation and reduced the chromosomal instability induced by FAA by 80%. Together these results confirm and extend the previously reported genetic instability occurring in cells from HT1 patients and allow us to speculate that this tumorigenic-related phenomenon may rely on the biochemical/cellular effects of FAA as a thiol-reacting and organelle/mitotic spindle-disturbing agent.
AuthorsR Jorquera, R M Tanguay
JournalHuman molecular genetics (Hum Mol Genet) Vol. 10 Issue 17 Pg. 1741-52 (Aug 15 2001) ISSN: 0964-6906 [Print] England
PMID11532983 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Acetoacetates
  • Reactive Oxygen Species
  • fumarylacetoacetate
  • Tyrosine
  • Protein-Serine-Threonine Kinases
  • Mitogen-Activated Protein Kinases
  • Mitogen-Activated Protein Kinase Kinases
  • Glutathione
Topics
  • Acetoacetates (metabolism)
  • Animals
  • Cell Line
  • Chromosome Aberrations
  • Cricetinae
  • Fluorescent Antibody Technique
  • G2 Phase (physiology)
  • Glutathione (metabolism)
  • Golgi Apparatus (metabolism, ultrastructure)
  • HeLa Cells
  • Humans
  • MAP Kinase Signaling System
  • Mitogen-Activated Protein Kinase Kinases (biosynthesis, genetics)
  • Mitogen-Activated Protein Kinases (metabolism)
  • Mitosis (physiology)
  • Phosphorylation
  • Protein-Serine-Threonine Kinases (biosynthesis, genetics)
  • Reactive Oxygen Species (metabolism)
  • Spindle Apparatus (metabolism)
  • Tyrosine (metabolism)
  • Tyrosinemias (genetics, metabolism)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research network!


Choose Username:
Email:
Password:
Verify Password: