[Clinical algorithm for cerebrospinal fluid test of 14-3-3 protein in diagnosis of Creutzfeldt-Jacob disease].

Abstract

OBJECTIVE:

To study whether an algorithm that includes additional diagnostic information could increase the specificity of the 14-3-3 protein testing in patients suspected to suffer from Creutzfeldt-Jakob disease (CJD).

DESIGN:

The development of a diagnostic algorithm.

METHOD:

The 14-3-3 protein was tested in the cerebrospinal fluid from 69 consecutive patients suspected of having CJD. On the basis of a former study and literature research, a diagnostic algorithm was constructed, which restricted the indication for performing the 14-3-3 protein test.

RESULTS:

By taking into consideration the findings of neuroimaging and routine cerebrospinal fluid examination prior to 14-3-3 testing, the specificity increased to 97% (95%-CI: 85.5-99.9) thus changing the prior probability of having CJD of 35% to a posterior probability of 75-100%, in the case of a positive test result.

CONCLUSION:

Determining the presence of 14-3-3 protein is a highly sensitive and specific marker for sporadic CJD when used in combination with imaging and cerebrospinal fluid examination.

• Authors: A W Lemstra, M van Meegen, F Baas, W A van Gool
• Journal: Nederlands tijdschrift voor geneeskunde (Ned Tijdschr Geneeskd) Vol. 145 Issue 30 Pg. 1467-71 (Jul 28 2001) ISSN: 0028-2162 [Print] Netherlands
• Vernacular Title: Een klinisch algoritme voor het gebruik van de 14-3-3-liquortest bij de diagnostiek van de ziekte van Creutzfeldt-Jakob.
• PMID: 11503317 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• 14-3-3 Proteins
• Biomarkers
• Tyrosine 3-Monooxygenase

Topics

• 14-3-3 Proteins
• Adolescent
• Adult
• Aged
• Aged, 80 and over
• Algorithms
• Biomarkers (cerebrospinal fluid)
• Child
• Creutzfeldt-Jakob Syndrome (cerebrospinal fluid, diagnosis)
• Diagnosis, Differential
• Female
• Humans
• Immunoblotting
• Male
• Middle Aged
• Sensitivity and Specificity
• Tyrosine 3-Monooxygenase (cerebrospinal fluid)