Cystatin C in a rat model of end-stage renal failure.

Abstract

BACKGROUND:

Cystatin C (MW 13kDa) serum concentration reflects glomerular filtration rate better than creatinine. Like other low-molecular weight proteins it is not eliminated by dialysis. Still, cystatin C serum concentrations do not rise progressively in end-stage renal failure and rarely exceed 10 mg/L (i.e. 8 times the upper limit of normal).

OBJECTIVE:

To study cystatin C kinetics in a rat model of end-stage renal failure.

METHODS:

Sequential bilateral nephrectomy was performed seven days apart in 13 male Sprague-Dawley rats as described by Levine and Saltzman. Serum cystatin C (Cystatin C PET-kit, DAKO), creatinine and total protein were measured in daily intervals after the second nephrectomy. Linearity of the anti-human cystatin C assay for rat cystatin C was tested using dilutions of uremic rat serum. Rats were sacrificed for signs of severe uremia on days 10 (n=5), 11 (n=4) and 12 (n = 5).

RESULTS:

At baseline, mean (+/- SE) cystatin C was 1.59+/-0.041 mg/L, creatinine 19.6+/-1.2 micromol/L. Following bilateral nephrectomy, cystatin C immediately rose to 3.82+/-0.15 mg/L, creatinine to 312+/-20 micromol/L. During the following days, cystatin C concentration stabilized to 4 mg/L approximately whereas creatinine continued to rise to 822+/-185 kmol/L on day 12. Correction for the decrease in serum total protein concentration from 48.9+/-2.3 g/L to 37.4+/-3.6 g/L did not alter these results.

CONCLUSION:

The kinetics of cystatin C and creatinine in this rat model of end-stage renal failure are in accordance with human data suggesting a change in cystatin C production or extra-renal elimination in severe chronic uremia.

• Authors: A Bökenkamp, G Ciarimboli, C Dieterich
• Journal: Renal failure (Ren Fail) 2001 May-Jul Vol. 23 Issue 3-4 Pg. 431-8 ISSN: 0886-022X [Print] England
• PMID: 11499558 (Publication Type: Journal Article)

Chemical References

• CST3 protein, human
• Cst3 protein, rat
• Cystatin C
• Cystatins
• Cysteine Proteinase Inhibitors
• Creatinine

Topics

• Animals
• Creatinine (blood)
• Cystatin C
• Cystatins (blood)
• Cysteine Proteinase Inhibitors (blood)
• Glomerular Filtration Rate
• Kidney Failure, Chronic (blood, metabolism)
• Male
• Models, Animal
• Nephrectomy
• Predictive Value of Tests
• Rats
• Rats, Sprague-Dawley