Interleukin-18 inhibits human immunodeficiency virus type 1 production in peripheral blood mononuclear cells.

Abstract

Interleukin (IL)-18 is an interferon (IFN)-gamma-inducing factor and contributes to the Th1 immune response. IL-18 added after infection of peripheral blood mononuclear cells (PBMC) with monocyte-tropic human immunodeficiency virus type 1 (HIV-1) inhibited p24 antigen production by a maximum of 72%. IFN-gamma levels in these cultures were increased, and a significant inverse relationship between HIV-1 production and IFN-gamma levels was observed. A neutralizing anti-IFN-gamma antibody reversed the IL-18 inhibitory effect. Preincubation of PBMC with IL-18 before infection inhibited p24 without additional IL-18 (64%). However, compared with the degree of IL-18 inhibition observed after a 4-day culture, no additional IL-18 inhibitory effect was observed during days 5-13. IL-18 also reduced cell surface expression of the HIV-1 receptor CD4. These results demonstrate that IL-18 inhibited HIV-1 production in PBMC through intermediate IFN-gamma. Furthermore, inhibition was present during the early stages of viral infection and was associated with reduced HIV-1 receptor expression.

• Authors: H J Choi, C A Dinarello, L Shapiro
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 184 Issue 5 Pg. 560-8 (Sep 01 2001) ISSN: 0022-1899 [Print] United States
• PMID: 11494162 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• CD4 Antigens
• HIV Core Protein p24
• Interleukin-18
• Interferon-gamma

Topics

• CD4 Antigens (metabolism)
• Cells, Cultured
• HIV Core Protein p24 (metabolism)
• HIV Infections (virology)
• HIV-1 (drug effects, physiology)
• Humans
• Interferon-gamma (biosynthesis)
• Interleukin-18 (pharmacology)
• Leukocytes, Mononuclear (virology)