Abstract |
The mechanism of body weight loss in the tumor-bearing state is still unclear. In this study, we investigated expressions of apoptosis regulatory proteins in the skeletal muscle of tumor-bearing and diet-restricted rabbits, and tried to evaluate the differences between the two groups. The apoptotic index (AI) in the tumor-bearing group was 28.1+/-2.84 on day 10. By day 20, many more apoptotic cells were found (AI: 40.5+/-3.20), but then after day 20 their numbers gradually decreased (AI: 9.67+/-2.22 on day 30 and 0.93+/-0.96 on day 40). By contrast, no apoptotic cells were detected in the diet-restricted group at any of the times examined. Bcl-2 immunoreactivity was either not detected at all or only weakly observed in both groups. By contrast, Bax expression increased gradually after implantation in the tumor-bearing group. Bax expression in skeletal muscle cell was graded (moderate) 10 days after tumor implantation, and (high) by day 20, in 2 of the 5 tumor-bearing rabbits. After day 20, however, Bax immunoreactivity decreased continuously in the tumor-bearing group. By contrast, hardly any Bax-immuno-positive cells were detected in the diet-restricted group. These results suggest that loss of body weight in the tumor-bearing group is different from that in the diet-restricted group, and is related to apoptosis of skeletal muscles.
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Authors | O Ishiko, T Sumi, H Yoshida, Y Hyun, S Ogita |
Journal | International journal of molecular medicine
(Int J Mol Med)
Vol. 8
Issue 3
Pg. 279-83
(Sep 2001)
ISSN: 1107-3756 [Print] Greece |
PMID | 11494056
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Proto-Oncogene Proteins
- Proto-Oncogene Proteins c-bcl-2
- bcl-2-Associated X Protein
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Topics |
- Adipose Tissue
- Animals
- Apoptosis
(genetics)
- Body Mass Index
- Body Weight
- DNA Fragmentation
(genetics)
- Diet, Reducing
- Immunohistochemistry
- In Situ Nick-End Labeling
- Male
- Muscle, Skeletal
(chemistry, metabolism, pathology)
- Neoplasm Transplantation
- Neoplasms, Experimental
(genetics, metabolism, physiopathology)
- Proto-Oncogene Proteins
(biosynthesis)
- Proto-Oncogene Proteins c-bcl-2
(biosynthesis)
- Rabbits
- Time Factors
- bcl-2-Associated X Protein
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