HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Preconditioning decreases ischemia/reperfusion-induced peroxynitrite formation.

Abstract
The role for peroxynitrite (ONOO(-)) in the mechanism of preconditioning is not known. Therefore, we studied effects of preconditioning and subsequent ischemia/reperfusion on myocardial ONOO(-) formation in isolated rat hearts. Hearts were subjected to a preconditioning protocol (three intermittent periods of global ischemia/reperfusion of 5 min duration each) followed by a test ischemia/reperfusion (30 min global ischemia and 15 min reperfusion). When compared to nonpreconditioned controls, preceding preconditioning improved postischemic cardiac performance and significantly decreased test ischemia/reperfusion-induced formation of free nitrotyrosine measured in the perfusate as a marker for cardiac endogenous ONOO(-) formation. During preconditioning, however, the first period of ischemia/reperfusion increased nitrotyrosine formation, which was attenuated after the third period of ischemia/reperfusion. We conclude that classic preconditioning inhibits ischemia/reperfusion-induced cardiac formation of ONOO(-) and that subsequent periods of ischemia/reperfusion result in a gradual attenuation of ischemia/reperfusion-induced ONOO(-) generation. This mechanism might be involved in ischemic adaptation of the heart.
AuthorsC Csonka, T Csont, A Onody, P Ferdinandy
JournalBiochemical and biophysical research communications (Biochem Biophys Res Commun) Vol. 285 Issue 5 Pg. 1217-9 (Aug 03 2001) ISSN: 0006-291X [Print] United States
PMID11478785 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
CopyrightCopyright 2001 Academic Press.
Chemical References
  • Nitrates
  • peroxynitric acid
  • 3-nitrotyrosine
  • Tyrosine
  • L-Lactate Dehydrogenase
Topics
  • Animals
  • Aorta
  • Blood Flow Velocity
  • In Vitro Techniques
  • Ischemic Preconditioning, Myocardial (methods)
  • L-Lactate Dehydrogenase (biosynthesis)
  • Male
  • Myocardial Ischemia (physiopathology)
  • Myocardial Reperfusion
  • Myocardial Reperfusion Injury (prevention & control)
  • Myocardium (metabolism)
  • Nitrates (metabolism)
  • Rats
  • Rats, Wistar
  • Time Factors
  • Tyrosine (analogs & derivatives, biosynthesis)
  • Ventricular Function, Left

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: