Abstract |
The role for peroxynitrite (ONOO(-)) in the mechanism of preconditioning is not known. Therefore, we studied effects of preconditioning and subsequent ischemia/reperfusion on myocardial ONOO(-) formation in isolated rat hearts. Hearts were subjected to a preconditioning protocol (three intermittent periods of global ischemia/reperfusion of 5 min duration each) followed by a test ischemia/reperfusion (30 min global ischemia and 15 min reperfusion). When compared to nonpreconditioned controls, preceding preconditioning improved postischemic cardiac performance and significantly decreased test ischemia/reperfusion-induced formation of free nitrotyrosine measured in the perfusate as a marker for cardiac endogenous ONOO(-) formation. During preconditioning, however, the first period of ischemia/reperfusion increased nitrotyrosine formation, which was attenuated after the third period of ischemia/reperfusion. We conclude that classic preconditioning inhibits ischemia/reperfusion-induced cardiac formation of ONOO(-) and that subsequent periods of ischemia/reperfusion result in a gradual attenuation of ischemia/reperfusion-induced ONOO(-) generation. This mechanism might be involved in ischemic adaptation of the heart.
|
Authors | C Csonka, T Csont, A Onody, P Ferdinandy |
Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 285
Issue 5
Pg. 1217-9
(Aug 03 2001)
ISSN: 0006-291X [Print] United States |
PMID | 11478785
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright 2001 Academic Press. |
Chemical References |
- Nitrates
- peroxynitric acid
- 3-nitrotyrosine
- Tyrosine
- L-Lactate Dehydrogenase
|
Topics |
- Animals
- Aorta
- Blood Flow Velocity
- In Vitro Techniques
- Ischemic Preconditioning, Myocardial
(methods)
- L-Lactate Dehydrogenase
(biosynthesis)
- Male
- Myocardial Ischemia
(physiopathology)
- Myocardial Reperfusion
- Myocardial Reperfusion Injury
(prevention & control)
- Myocardium
(metabolism)
- Nitrates
(metabolism)
- Rats
- Rats, Wistar
- Time Factors
- Tyrosine
(analogs & derivatives, biosynthesis)
- Ventricular Function, Left
|