HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Common germinal-center B-cell origin of the malignant cells in two composite lymphomas, involving classical Hodgkin's disease and either follicular lymphoma or B-CLL.

AbstractBACKGROUND:
Classical Hodgkin's disease (HD) and B-cell non-Hodgkin lymphoma (NHL) occasionally occur in the same patient. Such composite lymphomas represent interesting models to study the pathogenesis of B-cell lymphomas and the relationship between HD and B-cell NHL.
MATERIALS AND METHODS:
We analyzed two composite lymphomas (a combination of classical HD with follicular lymphoma [FL] and a combination of classical HD with B-cell chronic lymphocytic leukemia [B-CLL]) by micromanipulation of single cells from tissue sections and amplification of immunoglobulin V region genes for the clonal relationship of the tumor cells.
RESULTS:
In both cases, clonally related variable (V) genes with both shared as well as distinct somatic mutations were obtained from the two lymphomas, showing that in each of the cases the distinct tumor cells were members of a common germinal center (GC) B-cell clone. FL cells from two different lymph nodes of patient 1 showed a similar mutation pattern, suggesting that infiltration of these lymph nodes by tumor cells was not restricted to a particular FL cell or subclone. In the FL, a single cell was identified with a mutation signature indicating that premalignant cells can persist in the tissue.
CONCLUSIONS:
The cases presented here further underline the close relationship between HD and B-cell NHL and the role of the GC in lymphomagenesis. Whereas the latter was already suggested for FL and HD, the present study indicates that also in the B-CLL subset characterized by mutated Ig genes, important steps in malignant transformation happen in the GC, and that HRS cells can derive from CD5-positive B cells.
AuthorsR Küppers, A B Sousa, A S Baur, J G Strickler, K Rajewsky, M L Hansmann
JournalMolecular medicine (Cambridge, Mass.) (Mol Med) Vol. 7 Issue 5 Pg. 285-92 (May 2001) ISSN: 1076-1551 [Print] England
PMID11474574 (Publication Type: Case Reports, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Immunoglobulin Variable Region
Topics
  • Aged
  • Aged, 80 and over
  • Cell Lineage
  • Clone Cells
  • Fatal Outcome
  • Female
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain
  • Gene Rearrangement, B-Lymphocyte, Light Chain
  • Genes, Immunoglobulin (genetics)
  • Germinal Center (immunology, pathology)
  • Hodgkin Disease (genetics, pathology)
  • Humans
  • Immunoglobulin Variable Region (genetics)
  • Immunohistochemistry
  • Immunophenotyping
  • Leukemia, B-Cell (genetics, pathology)
  • Lymphoma, Follicular (genetics, pathology)
  • Male
  • Middle Aged
  • Mutation
  • Neoplasms, Multiple Primary (pathology)
  • Polymerase Chain Reaction
  • Reed-Sternberg Cells (immunology, pathology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: