HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

The comparison of electron microscopy and scintigraphy in determining the protective effect of dimethylsulphoxide (DMSO) on ischemia/reperfusion injury through Pringle maneuver.

AbstractBACKGROUND/AIMS:
We investigated the role of the electron microscopy and hepatobiliary scintigraphy in determining the effect of DMSO (dimethysulphoxide) and ischemia/reperfusion injury in the liver after the Pringle maneuver.
METHODOLOGY:
Twenty-four rabbits were divided into the following groups; A: Control group, B: Pringle, C: 10 mg/kg DMSO, D: 1 g/kg DMSO + Pringle. Group A was considered as a control group and only laparotomy was applied. Group B was exposed to Pringle maneuver only. Group C was given 10 mg/kg of DMSO via the vena cava inferior. Group D was given 1 g/kg of DMSO. A clamp was fastened for the groups of B, C and D in the 30th minute of the Pringle maneuver and a biopsy was applied five minutes later. Fifteen minutes later a dynamic hepatobiliary scintigraphy was applied. From dynamic images, liver peak time and activity half time of the liver were obtained.
RESULTS:
It was found that liver peak time and liver activity half time values of the group B, C and D were significantly longer than group A. Liver peak time and liver activity half time values of group B was not different from group C. However, some values of group D were found to be significantly shorter than groups B and C. In the electron microscopy examination, only in group B were some specific degenerative changes observed in the sinusoids. We observed less irreversible changes in group C than in group B. On the other hand, the least irreversible changes were in group D.
CONCLUSIONS:
As a conclusion, while electron microscopy is regarded as the "gold standard", hepatobiliary scintigraphy may be thought of as an easily applicable method in determining the ischemic reperfusion injury in the clinical comparison of the protective agents.
AuthorsA R Hatipoglu, E Temiz, M Yüksel, Z Hoscoskun, I Coskun, G Hüseyinova
JournalHepato-gastroenterology (Hepatogastroenterology) 2001 May-Jun Vol. 48 Issue 39 Pg. 799-802 ISSN: 0172-6390 [Print] Greece
PMID11462927 (Publication Type: Comparative Study, Journal Article)
Chemical References
  • Liver Glycogen
  • Reactive Oxygen Species
  • Dimethyl Sulfoxide
Topics
  • Animals
  • Dimethyl Sulfoxide (pharmacology)
  • Dose-Response Relationship, Drug
  • Ischemia (diagnostic imaging, pathology, prevention & control)
  • Liver (blood supply, diagnostic imaging, pathology)
  • Liver Function Tests
  • Liver Glycogen (metabolism)
  • Liver Regeneration (drug effects)
  • Microscopy, Electron
  • Rabbits
  • Radionuclide Imaging
  • Reactive Oxygen Species (metabolism)
  • Reperfusion Injury (diagnostic imaging, pathology, prevention & control)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: