Uveitopathogenic sites in recoverin.

Abstract

PURPOSE:

This study was carried out in order to determine the most potent and novel uveitopathogenic sites of recoverin using synthetic peptides.

METHODS:

Several synthetic peptides containing the recoverin sequence plus adjuvants were injected into Lewis rats, and the uveitopathogenic sequence was defined, clinically, histologically, and immunologically.

RESULTS:

Peptides containing of amino acids 57-85 and 136-167 induced severe EAU, and the lowest doses to induce EAU were 20 microg and 10 microg, respectively. Lymphocyte proliferative reactions were also positive for peptides 57-85 and 136-167. The core sequences within the uveitopathogenic site were 65-79 and 153-164. Peptides of amino acids 65-79 within 57-85 and 149-167 within 136-167 were the smallest in the recoverin sequence, respectively, that could induce severe EAU.

CONCLUSION:

We found recoverin has some novel potent uveitopathogenic sites, 149-167. These findings of the uveitopathogenic sites in recoverin may lead to improved understanding of the pathogenesis of uveitis and the means to design specific treatment.

• Authors: M Ohkoshi, T Abe, N Satoh, A Nakajima, S Sakuragi
• Journal: Current eye research (Curr Eye Res) Vol. 22 Issue 4 Pg. 304-11 (Apr 2001) ISSN: 0271-3683 [Print] England
• PMID: 11462170 (Publication Type: Journal Article)

Chemical References

• Calcium-Binding Proteins
• Eye Proteins
• Immunodominant Epitopes
• Lipoproteins
• Nerve Tissue Proteins
• Peptide Fragments
• Rcvrn protein, rat
• Recoverin
• Hippocalcin

Topics

• Animals
• Antibody Formation
• Autoimmune Diseases (immunology, pathology)
• Blotting, Western
• Calcium-Binding Proteins (immunology)
• Enzyme-Linked Immunosorbent Assay
• Eye Proteins
• Female
• Hippocalcin
• Immunization
• Immunodominant Epitopes (immunology)
• Lipoproteins
• Lymphocyte Activation (immunology)
• Nerve Tissue Proteins
• Peptide Fragments (immunology)
• Pineal Gland (immunology, pathology)
• Rats
• Rats, Inbred Lew
• Recoverin
• Retina (immunology, pathology)
• T-Lymphocytes (immunology)
• Uveitis (immunology, pathology)