Abstract | BACKGROUND: METHODS: Human peripheral blood monocytes were harvested from eight healthy volunteers and were exposed to three different concentrations of titanium particles or to 500 ng/mL of lipopolysaccharide as a positive control. The same volunteers were then given pentoxifylline (400 mg, five times per day) for seven days. Their peripheral blood monocytes were again isolated and exposed to experimental conditions, and the TNF-alpha levels were measured. RESULTS: The peripheral blood monocytes from all eight volunteers showed a significant reduction in TNF-alpha release following oral treatment with pentoxifylline. This reduction was observed at exposures of 10(7) and 10(6) titanium particles/mL and in the lipopolysaccharide-treated group, but not at 10(5) particles/mL. CONCLUSIONS: To our knowledge, this is the first study to demonstrate the ability of an oral drug to decrease the release of TNF-alpha from human peripheral blood monocytes exposed ex vivo to particle debris. TNF-alpha is involved in the pathogenesis of osteolysis and subsequent loosening of total joint arthroplasty components. The ability to suppress the release of TNF-alpha in patients with a total joint replacement may help to control osteolysis and to reduce the development of aseptic loosening. This effect could increase implant longevity and decrease the need for revision arthroplasty.
|
Authors | P F Pollice, R N Rosier, R J Looney, J E Puzas, E M Schwarz, R J O'Keefe |
Journal | The Journal of bone and joint surgery. American volume
(J Bone Joint Surg Am)
Vol. 83
Issue 7
Pg. 1057-61
(Jul 2001)
ISSN: 0021-9355 [Print] United States |
PMID | 11451976
(Publication Type: Clinical Trial, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- Lipopolysaccharides
- Tumor Necrosis Factor-alpha
- Titanium
- Pentoxifylline
|
Topics |
- Administration, Oral
- Adult
- Analysis of Variance
- Cells, Cultured
- Dose-Response Relationship, Drug
- Enzyme-Linked Immunosorbent Assay
- Equipment Failure Analysis
- Female
- Humans
- Joint Prosthesis
- Lipopolysaccharides
(pharmacology)
- Male
- Monocytes
(drug effects, metabolism)
- Pentoxifylline
(administration & dosage)
- Probability
- Reference Values
- Titanium
(pharmacology)
- Tumor Necrosis Factor-alpha
(analysis, drug effects)
|